机构：[1]Department of Ophthalmology, Tongren Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai 200336[2]Department of Ophthalmology, Ruijin Hospital Affiliated Medical School[3]Shanghai Key Laboratory for Bone and Joint Diseases,Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital Affiliated Medical School,Shanghai Jiaotong University, Shanghai 200025, P.R. China
The A(2A), receptor (A(2A)AR) antagonist has been considered as an attractive option to improve the treatment of neurological disorders, and the function of A(2A)R antagonist may inhibit the release of glutamate and prevent neuron damage. The aim of the present study was to investigate whether SCH442416 can modulate the glutamate uptake in retinal Muller cells under increased hydrostatic pressure. The levels of glutamine synthetase (GS) and glutamate aspartate transporter (GLAST) were assessed in retinal Muller cells under 40 mmHg pressure for 24 h using reverse transcription-quantitative polymerase chain reaction and western blotting, and a glutamate uptake assay was performed using a scintillation counting method. Following treatment of the Muller cells with 100 nM SCH442416 under 40 mmHg pressure br 24 h, the mRNA and protein expression levels of GS and GLAST, and glutamate uptake activity were investigated. Under 40 mmHg pressure, the expression levels of OS and GLAST in the Muller cells, and glutamate uptake activity were significantly reduced. Treatment with SCH442416 significantly ameliorated the decreased expression levels of GS and GLAST, and improved the glutamate uptake activity in the retinal Muller cells exposed to 40 mmHg pressure, resulting in increased expression levels of GS and GLAST, and increased glutamate uptake activity in the Muller cells under pressure. These results suggested that SCH442416 may be a potential candidate as a beneficial neuroprotective agent for the treatment of glaucoma by accelerating the clearance of extracellular glutamate.
基金：
National Natural Science
Foundation of China (grant no. 81371014) and the Shanghai
‘Science and Technology Innovation Actio§n Plan’ Basic
Research Key Project (grant nos. 11JC1407700 and
11JC1407701).
第一作者机构：[1]Department of Ophthalmology, Tongren Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai 200336
共同第一作者：
通讯作者：
通讯机构：[2]Department of Ophthalmology, Ruijin Hospital Affiliated Medical School[3]Shanghai Key Laboratory for Bone and Joint Diseases,Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital Affiliated Medical School,Shanghai Jiaotong University, Shanghai 200025, P.R. China[*1]Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin Er Road, Shanghai 200025, P.R. China[*2]Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, 197 Ruijin No. 2 Road, Shanghai 200025, P.R. China
推荐引用方式(GB/T 7714)：
Li Yong,Liu Xiaohong,Huang Shouyue,et al.Effect of SCH442416 on glutamate uptake in retinal Muller cells at increased hydrostatic pressure[J].MOLECULAR MEDICINE REPORTS.2015,12(3):3993-3997.doi:10.3892/mmr.2015.3882.
APA：
Li, Yong,Liu, Xiaohong,Huang, Shouyue,Huang, Ping&Zhong, Yisheng.(2015).Effect of SCH442416 on glutamate uptake in retinal Muller cells at increased hydrostatic pressure.MOLECULAR MEDICINE REPORTS,12,(3)
MLA：
Li, Yong,et al."Effect of SCH442416 on glutamate uptake in retinal Muller cells at increased hydrostatic pressure".MOLECULAR MEDICINE REPORTS 12..3(2015):3993-3997
