机构：[1]Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Ophthalmol, Beijing 100730, Peoples R China[2]Peking Union Med Coll, Beijing 100730, Peoples R China[3]Chinese Peoples Liberat Army Gen Hosp, Int Med Ctr, Beijing, Peoples R China[4]Hebei Prov Ophthalm Hosp, Dept Ophthalmol, Shijiazhuang, Hebei, Peoples R China[5]Capital Med Univ,Beijing Tongren Hosp,Dept Ophthalmol,Beijing,Peoples R China临床科室眼科首都医科大学附属北京同仁医院首都医科大学附属同仁医院眼科(未分亚科)
Successful cases of treatment of Bevacizumab for preventing scar after trabeculectomy in glaucoma patients encourage us to explore its mechanism. In this study, we primarily isolated conjunctival fibroblast from rat. RT-PCR analysis for the cells implicated that conjunctival fibroblast expressed vascular endothelial growth factor (VEGF) and its receptors. Immunofluorescence staining also showed positive staining for VEGFR-1. Furthermore, growth of fibroblast was significantly inhibited by Bevacizumab at dose of 2.5 mg/ml. Real time PCR results showed after 48 h intervention of 2.5 mg/ml Bevacizumab, the mRNA expressions of VEGF and its receptors decreased compared to the control group (P < 0.05) and Bevacizumab also decreased expression of TGF beta 1 and TGF beta 2 (P < 0.05). In summary, our finding demonstrated that Bevacizumab could directly act on fibroblast and inhibit VEGF, TGF beta 1, and TGF beta 2 expression.
第一作者机构：[1]Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Ophthalmol, Beijing 100730, Peoples R China[2]Peking Union Med Coll, Beijing 100730, Peoples R China
通讯作者：
通讯机构：[1]Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Ophthalmol, Beijing 100730, Peoples R China[2]Peking Union Med Coll, Beijing 100730, Peoples R China
推荐引用方式(GB/T 7714)：
Cheng Gangwei,Xiang Hang,Yang Guoxing,et al.Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast[J].CELL BIOCHEMISTRY AND BIOPHYSICS.2015,73(1):45-50.doi:10.1007/s12013-015-0565-0.
APA：
Cheng, Gangwei,Xiang, Hang,Yang, Guoxing,Ma, Jianmin&Zhao, Jialiang.(2015).Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast.CELL BIOCHEMISTRY AND BIOPHYSICS,73,(1)
MLA：
Cheng, Gangwei,et al."Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast".CELL BIOCHEMISTRY AND BIOPHYSICS 73..1(2015):45-50
