AIM: To examine the effects of sulforaphane on fibrotic changes of transforming growth factor (TGF beta 2) induced human conjunctival fibroblast (HConFs). METHODS: HConFs were cultured and divided into control, TGF beta 2 (1 ng/mL), sulforaphane and TGF beta 2+sulforaphane groups. Cell viability and apoptosis were detected using the MTT and ApoTox-Glo Triplex assay. Cell migration was detected using scratch and Transwell assay. Real-time quantitative PCR method was used to evaluate mRNA expression of TGF beta 2, matrix metalloproteinase-2 (MMP2), myosin light chain kinase (MYLK), integrin alpha V, integrin alpha 5, fibronectin 1 and alpha-smooth muscle actin (alpha-SMA). The protein expression of alpha-SMA, p-PI3K, PI3K, p-Akt, and Akt were detected by Western blot. RESULTS: The proliferation of HConFs was significantly (P<0.05) suppressed by sulforaphane compared to control cells with the increase of the concentration and treatment time. Cell proliferation after 48h incubation was significantly reduced with 100 mu mol/L sulforaphane treatment by 17.53% (P<0.05). The Transwell assay showed sulforaphane decreased cell migration by 18.73% compared with TGF beta 2-induced HConF (P<0.05). TGF beta 2-induced the increasing expression of fibronectin, type I collagen and a-SMA, and the phosphorylation of PI3K and Akt were all significantly suppressed by sulforaphane pretreatment. CONCLUSION: Sulforaphane inhibits proliferation, migration, and synthesis of the extracellular matrix in HConFs, and inhibiting the PI3K/Akt signaling pathway. Sulforaphane could be a potential therapeutic drug for prevention of scar formation in filtering bleb after trabeculectomy.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81700813]; Beijing Municipal Administration of Hospitals Qingmiao Projects [QML20180205]; Priming Scientific Research Foundation for the Junior Researcher in Beijing Tongren Hospital
第一作者机构：[1]Capital Med Univ, Beijing Inst Ophthalmol, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, 17 Hougouhutong Rd, Beijing 100005, Peoples R China[2]Beijing Key Lab Ophthalmol & Visual Sci, 17 Hougouhutong Rd, Beijing 100005, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Inst Ophthalmol, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, 17 Hougouhutong Rd, Beijing 100005, Peoples R China[2]Beijing Key Lab Ophthalmol & Visual Sci, 17 Hougouhutong Rd, Beijing 100005, Peoples R China[*1]Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University ,Beijing Key Laboratory of Ophthalmology & Visual Sciences, 17 Hougouhutong Road, Beijing 100005, China.
推荐引用方式(GB/T 7714)：
Liu Han-Ruo,Xia Zi-Yao,Wang Ning-Li.Sulforaphane modulates TGF beta 2-induced conjunctival fibroblasts activation and fibrosis by inhibiting PI3K/Akt signaling[J].INTERNATIONAL JOURNAL OF OPHTHALMOLOGY.2020,13(10):1505-1511.doi:10.18240/ijo.2020.10.01.
APA：
Liu, Han-Ruo,Xia, Zi-Yao&Wang, Ning-Li.(2020).Sulforaphane modulates TGF beta 2-induced conjunctival fibroblasts activation and fibrosis by inhibiting PI3K/Akt signaling.INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,13,(10)
MLA：
Liu, Han-Ruo,et al."Sulforaphane modulates TGF beta 2-induced conjunctival fibroblasts activation and fibrosis by inhibiting PI3K/Akt signaling".INTERNATIONAL JOURNAL OF OPHTHALMOLOGY 13..10(2020):1505-1511
