机构：[1]Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China医技科室检验科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Department of Laboratory Medicine, Beijing Tsinghua ChangGung Hospital, Medical Centre, Tsinghua University, Beijing 102218, China.
In order to evaluate a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry (MAIDI-TOF MS) assay in screening vancomycin-resistant Enterococcus faecium, a total of 150 E. faecium clinical strains were studied, including 60 vancomycin-resistant E. faecium (VREF) isolates and 90 vancomycin-susceptible (VSEF) strains. Vancomycin resistance genes were detected by sequencing. E. faecium were identified by MALDI-TOF MS. A genetic algorithm model with ClinProTools software was generated using the spectra of 30 VREF isolates and 30 VSEF isolates. Using this model, 90 test isolates were discriminated between VREF and VSEF. The results show that all 60 VREF isolates carried the vanA gene. The performance of VREF detection by the genetic algorithm model of MALDI-TOF MS compared to the sequencing method was sensitivity = 80%, specificity = 90%, false-positive rate = 10%, false-negative rate = 10%, positive predictive value = 80%, negative predictive value = 90%. MALDI-TOF MS can be used as u screening test for discrimination between vanA-positive E. faecium and vanA-negative E. frecium.
基金：
Capital Health Research and Development of Special [2014-4-2055]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类|4 区物理
小类|4 区物理：原子、分子和化学物理4 区光谱学
最新[2023]版：
大类|4 区化学
小类|4 区物理：原子、分子和化学物理4 区光谱学
JCR分区：
出版当年[2012]版：
Q3SPECTROSCOPYQ4PHYSICS, ATOMIC, MOLECULAR & CHEMICAL
最新[2023]版：
Q3SPECTROSCOPYQ4PHYSICS, ATOMIC, MOLECULAR & CHEMICAL
第一作者机构：[1]Department of Laboratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China
通讯作者：
推荐引用方式(GB/T 7714)：
Wu Wei,Wang Li-jun,Sui Wen-jun,et al.Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in the screening of vanA-positive Enterococcus faecium[J].EUROPEAN JOURNAL OF MASS SPECTROMETRY.2014,20(6):461-465.doi:10.1255/ejms.1298.
APA：
Wu Wei,Wang Li-jun,Sui Wen-jun,Zhang Gui&Lu Xin-xin.(2014).Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in the screening of vanA-positive Enterococcus faecium.EUROPEAN JOURNAL OF MASS SPECTROMETRY,20,(6)
MLA：
Wu Wei,et al."Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry in the screening of vanA-positive Enterococcus faecium".EUROPEAN JOURNAL OF MASS SPECTROMETRY 20..6(2014):461-465
