机构：[1]Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China临床科室耳鼻咽喉-头颈外科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China
To investigate the role of CUG-binding protein 1 (CUGBP1) in human laryngeal cancer, we employed lentivirus-mediated short hairpin RNA (shRNA) to knockdown CUGBP1 expression in Hep-2 cells. Depletion of CUGBP1 remarkably inhibited the proliferation of Hep-2 cells. CUGBP1 knockdown induced cell cycle arrest in S phase, especially in the sub-G1 phase, representing apoptotic cells. Knockdown of CUGBP1 in Hep-2 cells markedly increased the expression of LIP and cleavage of PARP, which could contribute to apoptosis. Thus CUGBP1 has a critical role in modulating cell growth and apoptosis, and serves as a potential therapeutic target in laryngeal cancer.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81072204]
第一作者机构：[1]Department of Otolaryngology, Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China[2]Department of Otolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhou Yi,Ma Hongzhi,Fang Jugao,et al.Knockdown of CUG-binding protein 1 induces apoptosis of human laryngeal cancer cells[J].CELL BIOLOGY INTERNATIONAL.2014,38(12):1408-1414.doi:10.1002/cbin.10356.
APA：
Zhou, Yi,Ma, Hongzhi,Fang, Jugao,Lian, Meng,Feng, Ling&Wang, Ru.(2014).Knockdown of CUG-binding protein 1 induces apoptosis of human laryngeal cancer cells.CELL BIOLOGY INTERNATIONAL,38,(12)
MLA：
Zhou, Yi,et al."Knockdown of CUG-binding protein 1 induces apoptosis of human laryngeal cancer cells".CELL BIOLOGY INTERNATIONAL 38..12(2014):1408-1414
