Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oligomers and constructed a naive human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that specifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked immunosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study successfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.
基金：
National Natural Science Foundation of China (NSFC) [30600099]
第一作者机构：[1]Beijing Normal Univ, Minist Educ, Key Lab Cell Proliferat & Regulat Biol, Beijing 100875, Peoples R China[2]Southeast Univ, Minist Educ, Key Lab Dev Genes & Human Dis, Nanjing 210009, Jiangsu, Peoples R China
通讯作者：
通讯机构：[1]Beijing Normal Univ, Minist Educ, Key Lab Cell Proliferat & Regulat Biol, Beijing 100875, Peoples R China[2]Southeast Univ, Minist Educ, Key Lab Dev Genes & Human Dis, Nanjing 210009, Jiangsu, Peoples R China
推荐引用方式(GB/T 7714)：
Yuan Zuanning,Du Minge,Chen Yiwen,et al.Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers[J].NEURAL REGENERATION RESEARCH.2013,8(33):3107-3115.doi:10.3969/j.issn.1673-5374.2013.33.004.
APA：
Yuan, Zuanning,Du, Minge,Chen, Yiwen&Dou, Fei.(2013).Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers.NEURAL REGENERATION RESEARCH,8,(33)
MLA：
Yuan, Zuanning,et al."Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers".NEURAL REGENERATION RESEARCH 8..33(2013):3107-3115
