机构：[1]Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China华中科技大学同济医学院附属同济医院[2]Department of Nuclear Medicine, Xiamen First Hospital, Fujian Medical University, Xiamen 361003, China[3]Department of Nuclear Medicine, Tongren Hospital, Beijing 100730, China首都医科大学附属同仁医院
The purpose of this study was to screen peptides that can specifically bind to human hepatocellular carcinoma (hHCC) cells using phage display of random peptide library in order to develope a peptide-based carrier for the diagnosis or therapy of hHCC. A peptide 12-mer phage display library was employed and 4 rounds of subtractive panning were performed using the hHCC cell line HepG2 as the target. After panning, the phages that specifically bound to and internalized in hHCC cells were selected. The selected phages demonstrated highly specific affinity to HepG2 cells analyzed by ELISA and immunofluorescence analysis. 57.3% of the selected phage clones displayed repeated sequence FLLEPHLMDTSM, and 4 amino acid residues, FLEP were extremely conservative. Based on the sequencing results, a 16-mer peptide (WH-16) was synthesized. The competitive ELISA showed that the binding of the phage clones displayed sequence FLLEPHLMDTSM to HepG2 cells was efficiently inhibited by WH-16. Our findings indicate that cellular binding of phage is mediated via its displayed peptide and the synthesized 16-mer peptide may have the potential to be a delivery carrier in target diagnosis or therapy for hHCC.
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第一作者机构：[1]Department of Nuclear Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430030, China
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推荐引用方式(GB/T 7714)：
Zhu Xiaohua,Wu Hua,Luo Sha,et al.Screening and identification of a novel hepatocellular carcinoma cell binding peptide by using a phage display library[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES.2008,28(3):299-303.doi:10.1007/s11596-008-0316-1.
APA：
Zhu, Xiaohua,Wu, Hua,Luo, Sha,Xianyu, Zhiqun&Zhu, Dan.(2008).Screening and identification of a novel hepatocellular carcinoma cell binding peptide by using a phage display library.JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES,28,(3)
MLA：
Zhu, Xiaohua,et al."Screening and identification of a novel hepatocellular carcinoma cell binding peptide by using a phage display library".JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES 28..3(2008):299-303
