Background This study aimed to investigate the influence of maternal allergy on cord blood regulatory and effector T cells and to evaluate their role as a predictor of atopic dermatitis (AD) during the first 2yr of life. Methods Seventy motherinfant pairs were recruited in this prospective birth cohort study (21 allergic and 49 non-allergic mothers). Cord blood samples were collected and assayed for the percentage of regulatory T cells (Treg), interferon- (IFN-), and interleukin-4 (IL-4) producing T cells (Th1 and Th2, respectively) using flow cytometry. Experiments were undertaken to assess the function of cord blood CD4+CD25+CD127 Treg cells by cell proliferation and cytokine responses. Their offspring at the age of 2yr old were evaluated by dermatologists to determine whether they had AD. Results During the first 2yr of life, 15.7% of the children developed a physician-diagnosed AD. A significantly increased percentage of Th2 cell was observed in cord blood of newborns with maternal allergy. Treg/Th2 ratio significantly decreased among the offspring of allergic mothers. Treg cell-associated suppression of Th2 response was attenuated in Der p1-stimulated CD4+CD25 T cells from the offspring of allergic mothers. Children with reduced Th1/Th2 (p=0.001, OR=0.37) and Treg/Th2 (p=0.001, OR=0.47) ratio in cord blood had a higher risk of developing AD. Conclusion Maternal allergic status is associated with increased percentage of IL-4+CD4+ T cells and a reduced Treg/Th2 ratio in cord blood at their children's birth, which may predispose to an increased risk for developing AD.
基金：
National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [81025007]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30973282]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7102030]; Special Fund of Sanitation Elite Reconstruction of Beijing [2009-2-007]
第一作者机构：[1]Capital Med Univ, Beijing TongRen Hosp, Dept Obstet & Gynaecol, Beijing, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[2]Capital Med Univ, Dept Otolaryngol Head & Neck Surg, Beijing TongRen Hosp, Beijing, Peoples R China[3]Beijing Inst Otolaryngol, Key Lab Otolaryngol Head & Neck Surg, Minist Educ China, Beijing 100005, Peoples R China[4]Johns Hopkins Univ, Div Allergy & Clin Immunol, Sch Med, Johns Hopkins Asthma & Allergy Ctr, Baltimore, MD 21224 USA[*1]Division of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, 5501 Hopkins Bayview Circle, 2A-20a Baltimore, MD 21224, USA.[*2]Department of Otolaryngology Head and Neck Surgery, Beijing Institute of Otolaryngology, No. 17, HouGouHuTong, DongCheng District, Beijing100005, China.
推荐引用方式(GB/T 7714)：
Fu Yujing,Lou Hongfei,Wang Chengshuo,et al.T cell subsets in cord blood are influenced by maternal allergy and associated with atopic dermatitis[J].PEDIATRIC ALLERGY AND IMMUNOLOGY.2013,24(2):178-186.doi:10.1111/pai.12050.
APA：
Fu, Yujing,Lou, Hongfei,Wang, Chengshuo,Lou, Wei,Wang, Yang...&Zhang, Luo.(2013).T cell subsets in cord blood are influenced by maternal allergy and associated with atopic dermatitis.PEDIATRIC ALLERGY AND IMMUNOLOGY,24,(2)
MLA：
Fu, Yujing,et al."T cell subsets in cord blood are influenced by maternal allergy and associated with atopic dermatitis".PEDIATRIC ALLERGY AND IMMUNOLOGY 24..2(2013):178-186
