Objective: The objective of this study was to evaluate the role of p38 MAP-kinase (MAPK) pathway on CLC-3 expression after interleukin-4 (IL-4) induction in primary cultured human nasal epithelial cells (HNECs) from patients with allergic rhinitis (AR). Methods. Cultured HNECs from five patients with AR were treated with IL-4 (20 ng/mL) with or without SB203580, a selective inhibitor of p38 MAPK at different concentrations and durations. CLC-3 was detected in HNECs by immunohistochemistry and real-time quantitative reverse transcription-polymerase chain reaction. p38 MAPK and phosphorylated p38 MAPK (pp38 MAPK) was examined by Western blotting. Results: After exposure to SB203580, CLC-3 expression induced by IL-4 was downregulated in HNECs in a concentration and time-dependent manner. This downregulation was associated with a decrease in pp,38 MAPK. Conclusion: These results confirmed that IL-4 can induce CLC-3 production in HNECs with AR through a p38 MAPK-dependent pathway. Inhibitors of p38 MAPK may become an important strategy for the treatment of AR.
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2005]版：
大类|4 区医学
最新[2023]版：
大类|3 区医学
小类|2 区耳鼻喉科学3 区医学：研究与实验
JCR分区：
出版当年[2004]版：
Q1OTORHINOLARYNGOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
最新[2023]版：
Q1OTORHINOLARYNGOLOGYQ3MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[2]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, Beijing, Peoples R China
通讯作者：
通讯机构：[1]Nanjing Med Univ, Affiliated Hosp 1, Dept Otolaryngol, Nanjing 210025, Peoples R China[2]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, Beijing, Peoples R China
推荐引用方式(GB/T 7714)：
Han Demin,Zhou Bing,Cheng Lei,et al.P38 MAP-kinase pathway is involved in the production of CLC-3 in nasal epithelial cells with allergic rhinitis induced by interleukin-4[J].LARYNGOSCOPE.2006,116(11):1973-1977.doi:10.1097/01.mlg.0000236078.81313.c8.
APA：
Han, Demin,Zhou, Bing,Cheng, Lei,Oh, Yun&Li, Huabin.(2006).P38 MAP-kinase pathway is involved in the production of CLC-3 in nasal epithelial cells with allergic rhinitis induced by interleukin-4.LARYNGOSCOPE,116,(11)
MLA：
Han, Demin,et al."P38 MAP-kinase pathway is involved in the production of CLC-3 in nasal epithelial cells with allergic rhinitis induced by interleukin-4".LARYNGOSCOPE 116..11(2006):1973-1977
