机构：[1]Uveitis Study Center, Sun Yal-sen University ,Key Laboratory of Ophthalmology，Sun Yat- sen University， Ministry of Education ,Zhongshan Ophthalmic Center ,Guangzhou 510060， China[2]Department of Ophthalmology, Beijing Tongren Eye Center, Beiing Tongren Hospital, Capital Medical University， Bejing 100730, China临床科室眼科眼科首都医科大学附属北京同仁医院首都医科大学附属同仁医院
Background Anterior chamber associated immune deviation (ACAID) is characterized by a Th-2 cell response. GATA-3 has been shown to be necessary for the activation of Th-2 cells. This study was designed to examine the expression of GATA-3 in the development of ACAID. Methods ACAID was induced by injection of 50 mu g interphotoreceptor retinoid binding protein ( IRBP) into the anterior chamber (AC) of Wistar rats. Delayed-type hypersensitivity (DTH) was evaluated on day 3, 7, 14, 21, 28 after IRBP inoculation. GATA-3 expression was detected using immunohistochemical staining. The expression of GATA-3 mRNA at different time points after AC injection of IRBP was assayed by reverse transcriptase polymerase chain reaction (RT-PCR). Results A significant DTH reaction was observed in Wistar rats on day 3 and 5 after IRBP inoculation. The DTH reaction was decreased 7 days after TRBP inoculation. GATA-3 expression was weak at both mRNA and protein levels in the normal spleen, but was significantly increased on day 5, 7, 14, and 21 after AC injection of IRBP. Conclusion The expression of GATA-3 is increased during ACAID, suggesting that GATA-3 may be involved in the development of ACAID.
基金：
Innovative Research Groups of China(No．30321004)and Guangdong Famous Doctor Project(No．2004—199)．
第一作者机构：[1]Uveitis Study Center, Sun Yal-sen University ,Key Laboratory of Ophthalmology，Sun Yat- sen University， Ministry of Education ,Zhongshan Ophthalmic Center ,Guangzhou 510060， China[2]Department of Ophthalmology, Beijing Tongren Eye Center, Beiing Tongren Hospital, Capital Medical University， Bejing 100730, China
通讯作者：
推荐引用方式(GB/T 7714)：
Fu T,Yang PZ,Huang XK,et al.GATA-3 expression in the development of anterior chamber associated immune deviation[J].CHINESE MEDICAL JOURNAL.2005,118(23):2000-2004.
APA：
Fu, T,Yang, PZ,Huang, XK,Huang, Q,Zhou, HY...&Chen, X.(2005).GATA-3 expression in the development of anterior chamber associated immune deviation.CHINESE MEDICAL JOURNAL,118,(23)
MLA：
Fu, T,et al."GATA-3 expression in the development of anterior chamber associated immune deviation".CHINESE MEDICAL JOURNAL 118..23(2005):2000-2004
