The aim of this study was to test the diagnostic feasibility of the polymerase chain reaction (PCR) for detection of infections with Chlamydia trachomatis in eye swabs from patients with conjunctivitis, and to establish the basic technique of the PCR for epidemiological survey. The results of the PCR were compared with the Mikro Trak immunofluorescence assay (IFA). From 49 specimens of patients with conjunctivitis, 31 were found positive by PCR (63%) and 23 by IFA (47%). On the other hand, in 10 normal eye specimens and 10 non-Chlamydia trachoma conjunctivitis specimens no Chlamydia trachomatis was detected.
第一作者机构:[*1]BEIJING INST OPHTHALMOL,17 HOU GOU LANE,BEIJING 100005,PEOPLES R CHINA
通讯作者:
通讯机构:[*1]BEIJING INST OPHTHALMOL,17 HOU GOU LANE,BEIJING 100005,PEOPLES R CHINA
推荐引用方式(GB/T 7714):
FAN J,ZHANG WH,WU YY,et al.DETECTION OF INFECTIONS OF THE EYE WITH CHLAMYDIA-TRACHOMATIS BY THE POLYMERASE CHAIN-REACTION[J].INTERNATIONAL OPHTHALMOLOGY.1993,17(6):327-330.
APA:
FAN, J,ZHANG, WH,WU, YY,JING, XY&CLAAS, ECJ.(1993).DETECTION OF INFECTIONS OF THE EYE WITH CHLAMYDIA-TRACHOMATIS BY THE POLYMERASE CHAIN-REACTION.INTERNATIONAL OPHTHALMOLOGY,17,(6)
MLA:
FAN, J,et al."DETECTION OF INFECTIONS OF THE EYE WITH CHLAMYDIA-TRACHOMATIS BY THE POLYMERASE CHAIN-REACTION".INTERNATIONAL OPHTHALMOLOGY 17..6(1993):327-330