The aim of this study was to evaluate riboflavin in rabbit corneas before and after corneal collagen cross-linking (CXL) using confocal laser scanning microscopy.A randomized controlled experimental study.The study was divided into 3 parts. In part 1 of this study, 30 eyes from 15 rabbits were used to evaluate riboflavin in de-epithelialized corneas before standard CXL. In part 2, 12 eyes from 6 rabbits were used to compare the differences of riboflavin concentration between standard CXL and transepithelial CXL (TE-CXL). In part 3, 12 eyes from another set of 6 rabbits were used to evaluate 1-day postoperative changes of riboflavin concentration between standard CXL and TE-CXL. Riboflavin concentrations in corneas were evaluated by calculating fluorescence densities with a confocal laser scanning microscope.In part 1, the riboflavin concentration after a 20-minute instillation was 0.036%, and did not reach the safety threshold (0.040%) for standard CXL. In part 2, the riboflavin concentration before TE-CXL was lower than 0.010%, which was significantly lower than standard CXL (P < 0.001). In part 3, corneal fluorescence decreased by approximately 100% (fluorescence quenching) 1 day after standard CXL and approximately 60% 1 day after TE-CXL.Application of 0.010% riboflavin solution for 30 minutes is essential for standard CXL. TE-CXL is not recommended because the epithelial layers are inadequately permeable to riboflavin during and 1 day after surgery.
基金：
National Natural Science Foundation of China
(no. 81070763, 81271041), the Beijing Natural Science Foundation (no.
7122041), and the Beijing Educational Committee Fund
(KZ201310025027).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
无
最新[2025]版：
大类|2 区医学
小类|2 区眼科学
第一作者：
第一作者机构：[1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing[2]Hebei Eye Hospital, Hebei Ophthalmology Key Lab, Xingtai City, Hebei Province, China.
通讯作者：
通讯机构：[1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Lab, Beijing[*1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Sciences Key Lab, No. 1 Dongjiaomin Xiang, Dongcheng District, Beijing 100730, China
推荐引用方式(GB/T 7714)：
Wang Mengmeng,Zhang Fengju,Zhao Xu,et al.Riboflavin Concentration Analysis in Rabbit Corneas Before and After Corneal Collagen Cross-Linking Using Confocal Laser Scanning Microscopy.[J].Asia-Pacific journal of ophthalmology (Philadelphia, Pa.).2014,3(6):388-94.doi:10.1097/APO.0000000000000100.
APA：
Wang Mengmeng,Zhang Fengju,Zhao Xu,Song Yanzheng&Zhang Miao.(2014).Riboflavin Concentration Analysis in Rabbit Corneas Before and After Corneal Collagen Cross-Linking Using Confocal Laser Scanning Microscopy..Asia-Pacific journal of ophthalmology (Philadelphia, Pa.),3,(6)
MLA：
Wang Mengmeng,et al."Riboflavin Concentration Analysis in Rabbit Corneas Before and After Corneal Collagen Cross-Linking Using Confocal Laser Scanning Microscopy.".Asia-Pacific journal of ophthalmology (Philadelphia, Pa.) 3..6(2014):388-94
