机构：[1]Department of Otolaryngology, Head and Neck Surgery, Beijing TongRenHospital, Capital Medical University, Beijing 100730, People’s Republic of China首都医科大学附属同仁医院[2]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology,Beijing 100005, People’s Republic of China研究所耳鼻咽喉科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Department of Allergy, BeijingTongRen Hospital, Capital Medical University, Beijing 100730, People’sRepublic of China临床科室变态反应科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[4]Research Unit of Diagnosis and Treatment of ChronicNasal Diseases, Chinese Academy of Medical Sciences, Beijing 100005, People’sRepublic of China
Background Eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP) exhibits a poorer outcome compared with non-eosinophilic chronic rhinosinusitis with nasal polyps (nonECRSwNP), so it is significant to identify effective markers to differentiate ECRSwNP in guiding the treatment strategies of these patients. Although arachidonate 15-lipoxygenase (ALOX15) is positioned as a marker of eosinophilic inflammation, its study in differentiating ECRSwNP has not been reported. The aim of this study is to assess the potential of ALOX15 in distinguishing and predicting ECRSwNP. Methods Forty-eight patients with chronic rhinosinusitis with nasal polyps (CRSwNP), including 30 ECRSwNP and 18 nonECRSwNP patients, were enrolled. ALOX15 mRNA level was determined in polyps by real-time polymerase chain reaction (RT-PCR). The patients' baseline characteristics were evaluated and analyzed for correlations with ALOX15. Receiver operating characteristic (ROC) curve was used to assess the predictive significance of the potential predictors for ECRSwNP. Results ALOX15 mRNA level was significantly higher in ECRSwNP patients than in nonECRSwNP patients (P < 0.001). ALOX15 mRNA was significantly correlated with tissue and blood eosinophil percentages (r = 0.565,P < 0.001 andr = 0.395,P = 0.006), olfaction scores (r = 0.400,P = 0.005), total visual analogue scale (VAS) symptom scores (r = 0.383,P = 0.007), ethmoid/maxillary sinus (E/M) ratio (r = 0.463,P = 0.001), and endoscopy scores (r = 0.409,P = 0.004). Logistic regression analysis showed ALOX15 mRNA level and percentage of blood eosinophils to be predictive factors for ECRSwNP (P = 0.004 andP = 0.036, respectively). ROC curve indicated ALOX15 to have high predictive accuracy for ECRSwNP (area under the curve (AUC) = 0.909), which was further improved by combination of ALOX15 with percentage of blood eosinophils (AUC = 0.933). Conclusions The relative ALOX15 mRNA level alone or in combination with blood eosinophils might be a reliable biomarker for predicting a diagnosis of ECRSwNP.
基金：
National Natural Science
Foundation of China (81870698, 81420108009, 81400444, 81470678
and 81630023), CAMS Innovation Fund for Medical Sciences (2019-I2M-
5-022), the National Key R&D Program of China (2018YFC0116801 and
2016YFC20160905200), the program for Changjiang Scholars and Innovative
Research Team (IRT13082), Beijing Municipal Administration of Hospitals’
Innovation Program of Clinical Techniques (XMLX201816), the Priming
Scientific Research Foundation for the Senior Researcher in Beijing TongRen
Hospital, Capital Medical University (2017-YJJ-GGL-005), Beijing Municipal
Administration of Hospitals incubating Program (PX20190007), Beijing Natural
Science Foundation (7194247), Public Welfare Development and Reform
Pilot Project (2019-10), and Beijing Scientific and Technological Overall Plan
(Z171100000117002).
第一作者机构：[1]Department of Otolaryngology, Head and Neck Surgery, Beijing TongRenHospital, Capital Medical University, Beijing 100730, People’s Republic of China[2]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology,Beijing 100005, People’s Republic of China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Otolaryngology, Head and Neck Surgery, Beijing TongRenHospital, Capital Medical University, Beijing 100730, People’s Republic of China[2]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology,Beijing 100005, People’s Republic of China[3]Department of Allergy, BeijingTongRen Hospital, Capital Medical University, Beijing 100730, People’sRepublic of China[4]Research Unit of Diagnosis and Treatment of ChronicNasal Diseases, Chinese Academy of Medical Sciences, Beijing 100005, People’sRepublic of China
推荐引用方式(GB/T 7714)：
Liang Zhuoping,Yan Bing,Liu Chang,et al.Predictive significance of arachidonate 15-lipoxygenase for eosinophilic chronic rhinosinusitis with nasal polyps[J].ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY.2020,16(1):doi:10.1186/s13223-020-00480-8.
APA：
Liang, Zhuoping,Yan, Bing,Liu, Chang,Tan, Ruyu,Wang, Chengshuo&Zhang, Luo.(2020).Predictive significance of arachidonate 15-lipoxygenase for eosinophilic chronic rhinosinusitis with nasal polyps.ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY,16,(1)
MLA：
Liang, Zhuoping,et al."Predictive significance of arachidonate 15-lipoxygenase for eosinophilic chronic rhinosinusitis with nasal polyps".ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY 16..1(2020)
