机构：[1]Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, China临床科室耳鼻咽喉-头颈外科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China研究所耳鼻咽喉科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases, Chinese Academy of Medical Sciences, Beijing, China[4]Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing, China临床科室变态反应科首都医科大学附属北京同仁医院首都医科大学附属同仁医院
Introduction: The recurrence occurs frequently among patients with chronic rhinosinusitis with nasal polyps (CRSwNP), and predictors that could be conveniently detected during practice in outpatient service are needed. Objective: We aimed to illustrate that the concentration of Charcot-Leyden crystal (CLC) in nasal secretions can effectively and noninvasively predict polyp recurrence. Methods: 108 patients with CRSwNP were divided into recurrence (n = 68) and recurrence-free (n = 40) groups. Preoperative CLC concentrations in nasal secretions were collected and detected by ELISA. Polyp tissues were harvested during biopsy or endoscopic sinus surgery and were evaluated for inflammatory cells by histopathological staining. Demographic information and the clinical characteristics of each patient were reviewed for associations with recurrence. Binary logistic regression analysis was performed to determine predictive factors for polyp recurrence. Receiver operating characteristic (ROC) curves and the Youden index were performed to determine their predictive values. Survival analysis was performed to compare recurrence risk of patients with different CLC concentrations. Results: Sixty-eight (62.96%) patients developed recurrence during a 12- to 33-month postoperative follow-up. CLC concentrations in nasal secretions were positively correlated with eosinophil percent in polyp tissue and peripheral blood and were significantly higher in patients of the recurrence group than in the patients of the recurrence-free group (p < 0.001). Binary logistic regression and ROC curve demonstrated that CLC protein in nasal secretions is predictive of polyp recurrence. According to the Youden index, a CLC concentration of 34.24 ng/mL can predict postoperative polyp recurrence with 92.6% sensitivity and 87.5% specificity. Patients with CLC concentrations higher than the cutoff value yielded a higher risk of recurrence (p < 0.001, HR = 11.31, 95% CI: 6.41-19.98). Conclusions: CLC protein in nasal secretions may serve as a promising noninvasive biomarker to predict CRSwNP recurrence. (C) 2020 S. Karger AG, Basel
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81900917, 81630023, 81870698]; CAMS Innovation Fund for Medical Sciences [2019-I2M-5-022]; Program for Changjiang Scholars and Innovative Research TeamProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13082]; National Key R&D Program of China [2018YFC0116800, 2016YFC20160905200]; Research of Capital Health Security and Cultivation [Z18110700160000]; Beijing Municipal Administration of Hospitals' Mission Plan [SML20150203]; Beijing Municipal Administration of Hospitals' Innovation Program of Clinical Techniques [XMLX201816]; Priming Scientific Research Foundation for the Senior Researcher in Beijing TongRen Hospital, Capital Medical University [2017-YJJGGL-005]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7194247]; Beijing Municipal Administration of Hospitals Incubating Program [PX20190007]; Public Welfare Development and Reform Pilot Project [2019-10]; Beijing Municipal Administration of Hospitals' Youth Programme [QML20150202]
第一作者机构：[1]Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, China[2]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China[3]Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases, Chinese Academy of Medical Sciences, Beijing, China[4]Department of Allergy, Beijing TongRen Hospital, Capital Medical University, Beijing, China[*1]Beijing TongRen Hospital, Capital Medical University, No. 1, DongJiaoMinXiang, Beijing 100730 (China)
推荐引用方式(GB/T 7714)：
Wu Di,Yan Bing,Wang Yang,et al.Predictive Significance of Charcot-Leyden Crystal Protein in Nasal Secretions in Recurrent Chronic Rhinosinusitis with Nasal Polyps[J].INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY.2021,182(1):65-75.doi:10.1159/000510120.
APA：
Wu, Di,Yan, Bing,Wang, Yang,Zhang, Luo&Wang, Chengshuo.(2021).Predictive Significance of Charcot-Leyden Crystal Protein in Nasal Secretions in Recurrent Chronic Rhinosinusitis with Nasal Polyps.INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY,182,(1)
MLA：
Wu, Di,et al."Predictive Significance of Charcot-Leyden Crystal Protein in Nasal Secretions in Recurrent Chronic Rhinosinusitis with Nasal Polyps".INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY 182..1(2021):65-75
