机构：[1]Nankai University School of Medicine, Tianjin, China.[2]The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, the College of Life Sciences, Tianjin, China.[3]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China.首都医科大学附属北京同仁医院首都医科大学附属同仁医院[4]State Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing 100853, China.[5]Jiangxi Engineering Research Center for Stem Cell, Shangrao, Jiangxi, China.[6]Tianjin Key Laboratory of Engineering Technologies for Cell Pharmaceutical, National Engineering Research Center of Cell Products, AmCellGene Co., Ltd., Tianjin, China.[7]Beijing Engineering Laboratory of Perinatal Stem Cells, Beijing Institute of Health and Stem Cells, Health & Biotech Co, Beijing, China.
Background Chemotherapy is an effective anti-tumor treatment. Mesenchymal stem cells (MSCs), exerting therapy effect on injured tissues during chemotherapy, may be damaged in the process. The possibility of self-healing through long-range paracrine and the mechanisms are unclear. Methods Doxorubicin, a commonly used chemotherapy drug, was to treat human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) for 6 h as an in vitro cell model of chemotherapy-induced damage. Then we use extracellular vesicles derived from placental mesenchymal stem cells (hP-MSCs) to investigate the therapeutic potential of MSCs-EVs for chemotherapy injury. The mechanism was explored using microRNA sequencing. Results MSC-derived extracellular vesicles significantly alleviated the chemotherapy-induced apoptosis. Using microRNA sequencing, we identified hsa-miR-11401, which was downregulated in the Dox group but upregulated in the EV group. The upregulation of hsa-miR-11401 reduced the expression of SCOTIN, thereby inhibiting p53-dependent cell apoptosis. Conclusions Hsa-miR-11401 expressed by MSCs can be transported to chemotherapy-damaged cells by EVs, reducing the high expression of SCOTIN in damaged cells, thereby inhibiting SCOTIN-mediated apoptosis.
基金：
This work was supported by grants from the National Natural Science
Foundation of China (32070860, 31771636, 81671734), National Key R&D Plan
(2017YFA0103201), Tianjin Natural Science Foundation (18YFZCSY00010,
18JCYBJC24400), Funds of State Key Laboratory of Kidney Diseases in PLA
General Hospital (KF-19-07), Postdoctoral Innovative Talent 799 Support
Foundation (BX20190382), and National Students’ Platform for Innovation
and Entrepreneurship Training Program (202010055095, 202010055845).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|2 区医学
小类|2 区医学：研究与实验3 区细胞与组织工程3 区细胞生物学
最新[2025]版：
大类|2 区医学
小类|2 区细胞与组织工程2 区细胞生物学2 区医学：研究与实验
JCR分区：
出版当年[2019]版：
Q1MEDICINE, RESEARCH & EXPERIMENTALQ2CELL & TISSUE ENGINEERINGQ2CELL BIOLOGY
最新[2023]版：
Q1CELL & TISSUE ENGINEERINGQ1CELL BIOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Nankai University School of Medicine, Tianjin, China.[2]The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, the College of Life Sciences, Tianjin, China.
通讯作者：
通讯机构：[1]Nankai University School of Medicine, Tianjin, China.[2]The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, the College of Life Sciences, Tianjin, China.
推荐引用方式(GB/T 7714)：
Li Huifang,Huang Haoyan,Chen Xiaoniao,et al.The delivery of hsa-miR-11401 by extracellular vesicles can relieve doxorubicin-induced mesenchymal stem cell apoptosis[J].STEM CELL RESEARCH & THERAPY.2021,12(1):doi:10.1186/s13287-021-02156-5.
APA：
Li, Huifang,Huang, Haoyan,Chen, Xiaoniao,Chen, Shang,Yu, Lu...&Li, Zongjin.(2021).The delivery of hsa-miR-11401 by extracellular vesicles can relieve doxorubicin-induced mesenchymal stem cell apoptosis.STEM CELL RESEARCH & THERAPY,12,(1)
MLA：
Li, Huifang,et al."The delivery of hsa-miR-11401 by extracellular vesicles can relieve doxorubicin-induced mesenchymal stem cell apoptosis".STEM CELL RESEARCH & THERAPY 12..1(2021)
医学