Objectives: The MeltPro MTB assays for detection of resistance to antituberculosis (TB) drugs perform well in genotypic drug susceptibility testing (DST) of clinical samples, but their effectiveness with formalin 7 fixed, paraffin-embedded (FFPE) tissues is unknown. Methods: FFPE tissues were obtained from 334 patients with TB. Susceptibility to rifampicin (RIF), isoniazid (INH), andfluoroquinolones was examined using the MeltPro MTB assays, with Xpert MTBIRIF (Xpert) andl or phenotypic DST (pDST) results as references. Samples with discordant results were analyzed by multiplex polymerase chain reaction-targeted amplicon sequencing (MTA-seq). Results: With pDST as the reference, the MeltPro MTB assays sensitivity for RIF, INH, levofloxacin (LVX), and moxifloxacin (MXF) was 95.00%, 96.00%, 100%, and 100%, respectively, and the specificity was 95.15%, 95.92%, 94.69%, and 89.92%, respectively. Concordance was 99.08% between the MeltPro MTB and Xpert (K = 0.956) for RIF and 95.12% (K = 0.834), 95.93% ( K = 0.880), 95.12% (K = 0.744), and 90.24% (K = 0.367) between the MeltPro MTB and pDST for RIP: INH, LVX, and MXE respectively. MTA-seq confirmed the discordancy between the MeltPro MTB and pDST for 26 (89.66%) of 29 samples. Conclusions: The MeltPro MTB assays rapidly and efficiently predict Mycobacterium tuberculosis resistance to the main first- and second-line anti-TB drugs in FFPE tissues.
基金：
Beijing Municipal Administration of
Hospitals’ Ascent Plan (grant DFL20181601) and Beijing Chest
Hospital, Capital Medical University/Beijing Tuberculosis and
Thoracic Tumor Research Institute Nurturing Project (2018‐7).
第一作者机构：[1]Capital Med Univ, Beijing TongRen Hosp, Dept Pathol, Key Lab Head & Neck Mol Diag Pathol, Beijing, Peoples R China[2]Capital Med Univ, Beijing Chest Hosp, Beijing TB & Thorac Tumor Res Inst, Dept Pathol, Beijing, Peoples R China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Mu Jing,Liu Zichen,Zhang Chen,et al.Performance of the MeltPro MTB Assays in the Diagnosis of Drug-Resistant Tuberculosis Using Formalin-Fixed, Paraffin-Embedded Tissues[J].AMERICAN JOURNAL OF CLINICAL PATHOLOGY.2021,156(1):34-41.doi:10.1093/ajcp/aqaa203.
APA：
Mu, Jing,Liu, Zichen,Zhang, Chen,Wang, Chongli,Du, Weili...&Liu, Honggang.(2021).Performance of the MeltPro MTB Assays in the Diagnosis of Drug-Resistant Tuberculosis Using Formalin-Fixed, Paraffin-Embedded Tissues.AMERICAN JOURNAL OF CLINICAL PATHOLOGY,156,(1)
MLA：
Mu, Jing,et al."Performance of the MeltPro MTB Assays in the Diagnosis of Drug-Resistant Tuberculosis Using Formalin-Fixed, Paraffin-Embedded Tissues".AMERICAN JOURNAL OF CLINICAL PATHOLOGY 156..1(2021):34-41
