Assessment of changes in genetic transcriptome in nasal epithelial cells exposed to ozone-aged black carbon and pollen allergen by high-throughput transcriptomics
机构：[1]Department of Allergy, Beijing Tongren Hospital, Capital Universityof Medical Science, Beijing 100730, China首都医科大学附属北京同仁医院临床科室变态反应科[2]Department of OtolaryngologyHead and Neck, Beijing Tongren Hospital, Capital University of MedicalScience, Beijing 100730, China临床科室耳鼻咽喉-头颈外科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Beijing Key Laboratory of Nasal Diseases,Beijing Institute of Otolaryngology, No.17, HouGouHuTong, DongchengDistrict, Beijing 100005, China首都医科大学附属北京同仁医院研究所耳鼻咽喉科研究所[4]Department of Environmental Medicine,Quanzhou Medical College, Quanzhou 362011, Fujian, China[5]Departmentof Biochemistry, Interdisciplinary Graduate School of Medicineand Engineering, University of Yamanashi, Yamanashi 409‑3898, Japan
Background Air pollution may be associated with increased airway responsiveness to allergens in allergic rhinitis (AR). Ozone-aged environmental black carbon (O3BC) is an important constituent of atmospheric particulate matter (PM), for which the mechanisms underlying its effects have not been fully elucidated in AR. The objective of the present study was to determine the O3BC and pollen-induced alterations in the transcriptome in human nasal epithelial cells (hNECs) in vitro. Methods hNECs from nasal epithelial mucosal samples of healthy individuals undergoing nasal surgery (turbinoplasty or septoplasty) were established as air-liquid interface (ALI) cultures and exposed to O3BC, pollen, or a combination of O3BC+ pollen. Changes in cell viability were analyzed by fluorescence and changes in the transcriptome by high-throughput RNA sequencing (RNA-seq). Several differentially expressed genes were verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Enrichment analysis, based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database, was performed to determine major biological functions and pathways involved. Results Exposure to >= 50 mu g/ml O3BC or 25 mu g/ml O3BC+ 200 mu g /ml pollen significantly decreased cell viability of the hNECs compared to control (p < 0.05) or 25 mu g/ml O3BC alone (p < 0.05); whereas exposure to pollen alone did not alter cell viability at any concentration investigated. High-throughput RNA sequencing analysis indicated that there was significant difference in gene expression between pollen or O3BC alone and O3BC+ pollen exposed cells. Exposure to 200 mu g/ml O3BC was associated with hypoxia stress response GO terms, whereas exposure to 25 mu g/ml O3BC+ 200 mu g/ml pollen was associated with inflammatory response GO terms; including regulation of neutrophil migration and chemotaxis, macrophage differentiation and chemotaxis, mast cell activation, and phagocytosis. KEGG pathway analysis indicated the top 10 upstream regulators to be IL1B, CSF1, CCL2, TLR2, LPL, IGF8, SPP1, CXCL8, FCER1G and IL1RN; of which expressions of inflammation-related genes IL1B, CSF1 and FCER1G were significantly increased. Conclusion O3BC and pollen allergen combined exposure may induce innate immune and allergic inflammation in hNECs, and therefore potentially exacerbate the symptoms of AR in affected individuals.
基金：
Beijing science and technology
commission science and technology planning project (Z191100009119013),
Beijing Municipal Science and Technology Project (Z181100001618002) and
the Beijing Health Bureau Program for High Level Talents (2014-3-017).
第一作者机构：[1]Department of Allergy, Beijing Tongren Hospital, Capital Universityof Medical Science, Beijing 100730, China[3]Beijing Key Laboratory of Nasal Diseases,Beijing Institute of Otolaryngology, No.17, HouGouHuTong, DongchengDistrict, Beijing 100005, China
通讯作者：
通讯机构：[1]Department of Allergy, Beijing Tongren Hospital, Capital Universityof Medical Science, Beijing 100730, China[2]Department of OtolaryngologyHead and Neck, Beijing Tongren Hospital, Capital University of MedicalScience, Beijing 100730, China[3]Beijing Key Laboratory of Nasal Diseases,Beijing Institute of Otolaryngology, No.17, HouGouHuTong, DongchengDistrict, Beijing 100005, China
推荐引用方式(GB/T 7714)：
Ouyang Yuhui,Li Ying,Xu Zhaojun,et al.Assessment of changes in genetic transcriptome in nasal epithelial cells exposed to ozone-aged black carbon and pollen allergen by high-throughput transcriptomics[J].ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY.2021,17(1):doi:10.1186/s13223-021-00553-2.
APA：
Ouyang, Yuhui,Li, Ying,Xu, Zhaojun,An, Yusan&Zhang, Luo.(2021).Assessment of changes in genetic transcriptome in nasal epithelial cells exposed to ozone-aged black carbon and pollen allergen by high-throughput transcriptomics.ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY,17,(1)
MLA：
Ouyang, Yuhui,et al."Assessment of changes in genetic transcriptome in nasal epithelial cells exposed to ozone-aged black carbon and pollen allergen by high-throughput transcriptomics".ALLERGY ASTHMA AND CLINICAL IMMUNOLOGY 17..1(2021)
