Porphyromonas gingivalis is one of the oral microorganisms associated with human chronic periodontitis. The purpose of this study is to determine the role of the receptor activator of nuclear factor-kappa B ligand (RANKL) in P. gingivalis infection-associated periodontal bone resorption. Inbred female Rowett rats were infected orally on four consecutive days (days 0 to 3) with 1 x 10(9) P. gingivalis bacteria (strain ATCC 33277). Separate groups of rats also received an injection of anti-RANKL antibody, osteoprotegerin fusion protein (OPG-Fc), or a control fusion protein (L6-Fc) into gingival papillae in addition to P. gingivalis infection. Robust serum IgG and salivary IgA antibody (P<0.01) and T cell proliferation (P<0.05) responses to P. gingivalis were detected at day 7 and peaked at day 28 in P. gingivalis-infected rats. Both the concentration of soluble RANKL (sRANKL) in rat gingival tissues (P<0.01) and periodontal bone resorption (P<0.05) were significantly elevated at day 28 in the P. gingivalis-infected group compared to levels in the uninfected group. Correspondingly, RANKL-expressing T and B cells in rat gingival tissues were significantly increased at day 28 in the P. gingivalis-infected group compared to the levels in the uninfected group (P<0.01). Injection of anti-RANKL antibody (P<0.05) or OPG-Fc (P<0.01), but not L6-Fc, into rat gingival papillae after P. gingivalis infection resulted in significantly reduced periodontal bone resorption. This study suggests that P. gingivalis infection-associated periodontal bone resorption is RANKL dependent and is accompanied by increased local infiltration of RANKL-expressing T and B cells.
基金：
NIH from the National Institute of Dental and Craniofacial Research [DE-03420, DE-07327]
基金编号：DE-03420 DE-07327
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2012]版：
大类|2 区医学
小类|2 区传染病学3 区免疫学
最新[2025]版：
大类|3 区医学
小类|3 区免疫学3 区传染病学
第一作者：
第一作者机构：[1]Forsyth Inst, Dept Immunol & Infect Dis, Cambridge, MA USA[*1]Forsyth Inst, Dept Immunol & Infect Dis, Cambridge, MA USA
通讯作者：
通讯机构：[1]Forsyth Inst, Dept Immunol & Infect Dis, Cambridge, MA USA[*1]Forsyth Inst, Dept Immunol & Infect Dis, Cambridge, MA USA
推荐引用方式(GB/T 7714)：
Han Xiaozhe,Lin Xiaoping,Yu Xiaoqian,et al.Porphyromonas gingivalis Infection-Associated Periodontal Bone Resorption Is Dependent on Receptor Activator of NF-kappa B Ligand[J].INFECTION AND IMMUNITY.2013,81(5):1502-1509.doi:10.1128/IAI.00043-13.
APA：
Han, Xiaozhe,Lin, Xiaoping,Yu, Xiaoqian,Lin, Jiang,Kawai, Toshihisa...&Taubman, Martin A..(2013).Porphyromonas gingivalis Infection-Associated Periodontal Bone Resorption Is Dependent on Receptor Activator of NF-kappa B Ligand.INFECTION AND IMMUNITY,81,(5)
MLA：
Han, Xiaozhe,et al."Porphyromonas gingivalis Infection-Associated Periodontal Bone Resorption Is Dependent on Receptor Activator of NF-kappa B Ligand".INFECTION AND IMMUNITY 81..5(2013):1502-1509
医学