机构：[1]Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China[2]Rutgers State Univ, Ctr Adv Biotechnol & Med, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA[3]Rutgers State Univ, Dept Pediat, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA[4]Univ Calif Irvine, Dept Med, Div Endocrinol & Metab, Irvine, CA 92697 USA[5]Univ Calif Irvine, Dept Biol Chem, Div Endocrinol & Metab, Irvine, CA 92697 USA
Understanding the molecular basis by which distinct cell types are specified is a central issue in retinogenesis and retinal disease development. Here we examined the role of LIM domain only 4 (Lmo4) in retinal development using both gain-of-function and loss-of-function approaches. By immunostaining, Lmo4 was found to be expressed in mouse retina from E10.5 to mature stages. Retroviral delivery of Lmo4 into retinal progenitor cells could promote the amacrine, bipolar and Muller cell fates at the expense of photoreceptors. It also inhibited the fate of early-born retinal ganglion cells. Using a dominant-negative form of Lmo4 which suppresses transcriptional activities of all LIM domain only factors, we demonstrated that LIM domain only factors are both necessary and sufficient for promoting amacrine and bipolar cell development, but not for the differentiation of ganglion, horizontal, Muller, or photoreceptor cells. Taken together, our study uncovers multiple roles of Lmo4 during retinal development and demonstrates the importance of LIM domain only factors in ensuring proper retinal cell specification and differentiation. (c) 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 900-915, 2016
基金：
Fundamental Research Funds of the State Key Laboratory of Ophthalmology, Sun Yat-sen University The National Basic Research Program (973 Program) of China [2015CB964600]; National Institutes of Health [EY020849, EY012020]
基金编号：2015CB964600 EY012020EY020849
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|2 区生物
小类|2 区发育生物学3 区神经科学
最新[2025]版：
大类|4 区医学
小类|4 区发育生物学4 区神经科学
第一作者：
第一作者机构：[1]Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China[2]Rutgers State Univ, Ctr Adv Biotechnol & Med, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA[3]Rutgers State Univ, Dept Pediat, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA[*1]Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China[*2]Rutgers State Univ, Ctr Adv Biotechnol & Med, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA[*3]Rutgers State Univ, Dept Pediat, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA
通讯作者：
通讯机构：[1]Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China[*1]Sun Yat Sen Univ, Zhongshan Ophthalm Ctr, State Key Lab Ophthalmol, Guangzhou 510060, Guangdong, Peoples R China[2]Rutgers State Univ, Ctr Adv Biotechnol & Med, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA[*2]Rutgers State Univ, Ctr Adv Biotechnol & Med, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA[3]Rutgers State Univ, Dept Pediat, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA[*3]Rutgers State Univ, Dept Pediat, Robert Wood Johnson Med Sch, Piscataway, NJ 08854 USA
推荐引用方式(GB/T 7714)：
Jin Kangxin,Xiao Dongchang,Andersen Bogi,et al.Lmo4 and Other LIM domain only factors are necessary and sufficient for multiple retinal cell type development[J].DEVELOPMENTAL NEUROBIOLOGY.2016,76(8):900-915.doi:10.1002/dneu.22365.
APA：
Jin, Kangxin,Xiao, Dongchang,Andersen, Bogi&Xiang, Mengqing.(2016).Lmo4 and Other LIM domain only factors are necessary and sufficient for multiple retinal cell type development.DEVELOPMENTAL NEUROBIOLOGY,76,(8)
MLA：
Jin, Kangxin,et al."Lmo4 and Other LIM domain only factors are necessary and sufficient for multiple retinal cell type development".DEVELOPMENTAL NEUROBIOLOGY 76..8(2016):900-915
