Activation of Toll-like receptor 9 inhibits lipopolysaccharide-induced receptor activator of nuclear factor kappa- B ligand expression in rat B lymphocytes
B lymphocytes express multiple TLRs that regulate their cytokine production. We investigated the effect of TLR4 and TLR9 activation on receptor activator of NF-kappa B ligand (RANKL) expression by rat spleen B cells. Splenocytes or purified spleen B cells from Rowett rats were cultured with TLR4 ligand Escherichia coli LPS and/or TLR9 ligand CpG-oligodeoxynucleotide (CpG-ODN) for 2 days. RANKL mRNA expression and the percentage of RANKL-positive B cells were increased in rat splenocytes challenged by E. coli LPS alone. The increases were less pronounced when cells were treated with both CpG-ODN and E. coli LPS. Microarray analysis showed that expressions of multiple cyclin-dependent kinase (CDK) pathway-related genes were up-regulated only in cells treated with both E. coli LPS and CpG-ODN. This study suggests that CpG-ODN inhibits LPS-induced RANKL expression in rat B cells via regulation of the CDK pathway.
基金：
NIH from the National Institute of Dental and Craniofacial Research [DE-003420, DE-021837]
基金编号： DE-021837DE-003420
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类|4 区医学
小类|4 区免疫学4 区微生物学
最新[2023]版：
大类|4 区医学
小类|4 区免疫学4 区微生物学
第一作者：
第一作者机构：[1]Forsyth Inst, Dept Immunol & Infect Dis, Cambridge, MA 02142 USA[2]Peking Univ Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
通讯作者：
通讯机构：[*1]Forsyth Inst, Dept Immunol & Infect Dis, 245 First St, Cambridge, MA 02142 USA
推荐引用方式(GB/T 7714)：
Yu Xiaoqian,Lin Jiang,Yu Qing,et al.Activation of Toll-like receptor 9 inhibits lipopolysaccharide-induced receptor activator of nuclear factor kappa- B ligand expression in rat B lymphocytes[J].MICROBIOLOGY AND IMMUNOLOGY.2014,58(1):51-60.doi:10.1111/1348-0421.12115.
APA：
Yu, Xiaoqian,Lin, Jiang,Yu, Qing,Kawai, Toshihisa,Taubman, Martin A.&Han, Xiaozhe.(2014).Activation of Toll-like receptor 9 inhibits lipopolysaccharide-induced receptor activator of nuclear factor kappa- B ligand expression in rat B lymphocytes.MICROBIOLOGY AND IMMUNOLOGY,58,(1)
MLA：
Yu, Xiaoqian,et al."Activation of Toll-like receptor 9 inhibits lipopolysaccharide-induced receptor activator of nuclear factor kappa- B ligand expression in rat B lymphocytes".MICROBIOLOGY AND IMMUNOLOGY 58..1(2014):51-60
