Circular RNAs (circRNAs) are class of non-coding RNAs formed by back-splicing events as loops, and could be found in all types of organisms. They play important and diverse roles in cell development, growth, and tumorigenesis, but functions of the majority of circRNAs remain enigmatic. Particularly functional phenotypes of great majority of circRNAs are not obvious. Here we randomly selected a circRNA circ-UBR5, which has no obvious functional phenotype in non-small cell lung cancer (NSCLC) cells from our previous research findings, to explore its potential function in cells. Differential expression of circ-UBR5 was detected in paired samples of tumorous tissues and adjacent nontumorous tissues from 59 patients with NSCLC by real-time quantitative reverse transcription-polymerase chain reactions (qRT-PCRs). Results showed circ-UBR5 expression was significantly downregulated in NSCLC tissues (p < 0.001) and was correlated with tumor differentiation (p = 0.00126), suggesting circ-UBR5 might serve as an index of NSCLC differentiation. Our findings indicated circ-UBR5 could bind splicing regulatory factor QKI, KH domain containing RNA binding (QKI) and NOVA alternative splicing regulator 1 (NOVA1) and U1 small nuclear RNA (snRNA) in the nucleus, revealing circ-UBR5 might be a novel snRNA involved in RNA splicing regulatory process. Moreover, we first presented a highly efficient strategy for finding specific circRNA binding proteins using Human Protein Microarray (HuprotTM Protoarray). (C) 2018 The Authors. Published by Elsevier Inc.
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出版当年[2017]版：
大类|3 区生物
小类|4 区生化与分子生物学4 区生物物理
最新[2025]版：
大类|4 区生物学
小类|4 区生化与分子生物学4 区生物物理
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第一作者机构：[1]Shanghai Jiao Tong Univ, Sch Med, 280 Chongqing South Rd, Shanghai 200025, Peoples R China[2]Fudan Univ, Shanghai Med Coll, 131 Dongan Rd, Shanghai 200032, Peoples R China[*1]Shanghai Jiao Tong Univ, Sch Med, 280 Chongqing South Rd, Shanghai 200025, Peoples R China
Qin Meilin,Wei Gang,Sun Xiaomeng.Circ-UBR5: An exonic circular RNA and novel small nuclear RNA involved in RNA splicing[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2018,503(2):1027-1034.doi:10.1016/j.bbrc.2018.06.112.
APA：
Qin, Meilin,Wei, Gang&Sun, Xiaomeng.(2018).Circ-UBR5: An exonic circular RNA and novel small nuclear RNA involved in RNA splicing.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,503,(2)
MLA：
Qin, Meilin,et al."Circ-UBR5: An exonic circular RNA and novel small nuclear RNA involved in RNA splicing".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 503..2(2018):1027-1034
