机构：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Natl Lab Med Mol Biol, Beijing 100005, Peoples R China[2]Peking Union Med Coll, Beijing 100005, Peoples R China[3]Hebei Med Univ, Hosp 4, Res Ctr, Shijiazhuang 050011, Hebei, Peoples R China[4]Harbin Med Univ, Affiliated Hosp 1, Dept Gen Surg, Harbin 150001, Heilongjiang, Peoples R China
Recently, long noncoding RNAs (lncRNAs) have been reported to play a pivotal role in the occurrence and progression of cancer because of their unique characteristics and have therefore become an active area of cancer research. The object of this study was to screen lncRNAs that are dysregulated in gastric cancer and to investigate their potential functions. Global expression of lncRNAs in gastric cancer and adjacent normal tissues of patients was profiled using a microarray assay. We identified an lncRNA (GALNT5 uaRNA, UTR-associated RNA) that is derived from the 3'-UTR of GALNT5. This lncRNA was transcribed independently of the coding region of GALNT5 and was determined to be markedly upregulated in human gastric carcinoma relative to their corresponding normal gastric tissues by quantitative RT-PCR (qRT-PCR) analysis of tissues from 122 gastric carcinoma patients. The expression of GALNT5 uaRNA was significantly correlated with the TNM stage and with lymph node metastasis. Further results demonstrated that GALNT5 uaRNA facilitated the proliferation and migration of gastric cancer cells in vitro and promoted tumor growth in a mouse model of human gastric cancer. Our results also indicated that GALNT5 uaRNA might function in gastric cancer by binding with HSP90. Further studies indicated that the 5'-end stem-loop motifs of GALNT5 uaRNA promoted the binding of HSP90 and its client proteins, and thus inhibited ubiquitination of the clients. These results expanded our understanding of GALNT5 uaRNA as a new avenue for therapeutic intervention against gastric cancer progression.
基金：
Natural Science Foundation of China [81572755, 81372200]; CAMS Initiative for Innovative Medicine (CAMS-I2M) [2016-I2M-1-001]
基金编号： 81372200815727552016-I2M-1-001
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|1 区医学
小类|1 区遗传学2 区生化与分子生物学2 区细胞生物学2 区肿瘤学
最新[2023]版：
大类|1 区医学
小类|1 区生化与分子生物学1 区遗传学2 区细胞生物学2 区肿瘤学
第一作者：
第一作者机构：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Natl Lab Med Mol Biol, Beijing 100005, Peoples R China[2]Peking Union Med Coll, Beijing 100005, Peoples R China
通讯作者：
通讯机构：[1]Chinese Acad Med Sci, Inst Basic Med Sci, Natl Lab Med Mol Biol, Beijing 100005, Peoples R China[*1]Chinese Acad Med Sci, Inst Basic Med Sci, Natl Lab Med Mol Biol, Beijing 100005, Peoples R China[2]Peking Union Med Coll, Beijing 100005, Peoples R China[*2]Peking Union Med Coll, Beijing 100005, Peoples R China[*3]Harbin Med Univ, Affiliated Hosp 1, Dept Gen Surg, Harbin 150001, Heilongjiang, Peoples R China[4]Harbin Med Univ, Affiliated Hosp 1, Dept Gen Surg, Harbin 150001, Heilongjiang, Peoples R China
推荐引用方式(GB/T 7714)：
Guo Hui,Zhao Lianmei,Shi Bianhua,et al.GALNT5 uaRNA promotes gastric cancer progression through its interaction with HSP90[J].ONCOGENE.2018,37(33):4505-4517.doi:10.1038/s41388-018-0266-4.
APA：
Guo, Hui,Zhao, Lianmei,Shi, Bianhua,Bao, Jiayu,Zheng, Dexian...&Shi, Juan.(2018).GALNT5 uaRNA promotes gastric cancer progression through its interaction with HSP90.ONCOGENE,37,(33)
MLA：
Guo, Hui,et al."GALNT5 uaRNA promotes gastric cancer progression through its interaction with HSP90".ONCOGENE 37..33(2018):4505-4517
医学