Purpose: In order to providing an experimental and theoretical bases for the clinical application of FR/NIR in myopia treatment, this work investigated the therapeutic efficacy and underlying mechanisms of far-red/nearinfrared light (FR/NIR) intervention in lens-induced myopia (LIM) progression using a LIM guinea pig model. Methods: In this study, 2-week-old healthy tricolor guinea pigs were randomly assigned to three groups: normal control (NC) group, LIM group and LIM treated with FR/NIR (LIM + FR/NIR) group. The guinea pigs in the LIM and LIM + FR/NIR groups wore a-6.00D lenses on the right eye to establish the LIM model. The guinea pigs in the LIM + FR/NIR group received FR/NIR treatment, while those in the NC group had no intervention. The right eyes were used to experimental study, and the experimental period was 3 weeks. Before and after the experiment, ocular parameters such as refractive status and axial length were measured. The related indexes of the retina and choroid were evaluated, and hematoxylin-eosin (HE) staining was performed to observe the changes in retinal morphological structure. Transcriptome sequencing, RT-qPCR, and immunofluorescence staining were employed to elucidate the cell and molecular mechanisms. Results: After 3 weeks of intervention, the LIM group developed a relative myopia shift of-6.83D, significantly higher than the-3.66 D shift in the LIM + FR/NIR group. Compared with the NC group, significant changes were observed in multiple ocular parameters of the LIM group. However, the progression was attenuated by FR/NIR treatment. The LIM + FR/NIR group owned the increased retinal/choroidal thickness, the enhanced choroidal blood perfusion, and the preserved retinal morphological structure that resembles to physiological norms. The results of transcriptome sequencing and related testament indicated that FR/NIR could regulate the expression of photoreceptor-related genes and improve photoreceptor function. Conclusion: FR/NIR can effectively suppresses myopia progression in LIM guinea pigs, maintain retinal/choroidal integrity, and ameliorate ischemic-hypoxic microenvironments. Its mechanism of action may involve regulating photoreceptor-related genes to improve photoreceptor function.
基金：
Foundation of Anhui Province [2208085J13]; Natural Science Foundation of Tibet Autonomous Region [XZ202501ZR0090]; Natural Science Foundation for Group-style Medical Aid Project of Tibet Autonomous Region [XZZR202402034 [W]]; Major Science and Technology Project of Zhongshan City of Guangdong Province on the Strategic Emerging Industries Technology Research Topic [2022A1007]; College Students' Innovation and Entrepreneurship Training Project of Anhui Province [S202410366013]
第一作者机构：[1]Anhui Med Univ, Affiliated Hosp 2, Dept Ophthalmol, 678 Furong Rd, Hefei 230601, Peoples R China
通讯作者：
通讯机构：[1]Anhui Med Univ, Affiliated Hosp 2, Dept Ophthalmol, 678 Furong Rd, Hefei 230601, Peoples R China[3]Hefei Univ Technol, Sch Mat Sci & Engn, Hefei, Peoples R China[4]Hefei Univ Technol, Intelligent Mfg Inst, Hefei, Peoples R China[5]Shannan Peoples Hosp Tibet, Dept Ophthalmol, Shannan 856100, Peoples R China
推荐引用方式(GB/T 7714)：
Ding Jingjing,Wang Xue,Cao Fan,et al.Therapeutic effect and mechanism of far-red/near-infrared light on lens-induced myopia Guinea pigs[J].BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS.2025,777:doi:10.1016/j.bbrc.2025.
APA：
Ding, Jingjing,Wang, Xue,Cao, Fan,Gao, Yeyan,Wang, Chuanxi...&Liu, Dongwei.(2025).Therapeutic effect and mechanism of far-red/near-infrared light on lens-induced myopia Guinea pigs.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,777,
MLA：
Ding, Jingjing,et al."Therapeutic effect and mechanism of far-red/near-infrared light on lens-induced myopia Guinea pigs".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 777.(2025)
