Myocardial infarction is a major cause of death and disability worldwide and poses a serious threat to global public health. Chronic ischemia and hypoxic environment induce excessive inflammatory response, leading to heart failure and even death. Therefore, inhibiting excessive inflammation and promoting tissue repair are important for myocardial repair. Based on this, natural polymeric Silk Fibroin Methacryloyl microneedles loaded with Tc peptide drugs (SilMA MNs) were fabricated for the MI therapy by photocuring and micro-molding techniques, which facilitate the protection of Tc peptides that can be rapidly degraded in physiological environments. SilMA MNs containing Tc could promote proliferation and spreading of AC16 and H9C2 cardiomyocytes, alleviate excessive inflammation of macrophages under LPS induction, and further migration and angiogenesis of HUVECs endothelial cell, showing the potential for MI treatment in clinical applications.