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CircSAMD4A aggravates H/R-induced cardiomyocyte apoptosis and inflammatory response by sponging miR-138-5p.

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机构： [1]Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan, China. [2]Department of Cardiology, Tongren Hospital of Wuhan University, Wuhan, China. [3]Department of Emergency, Renmin Hospital of Wuhan University, Wuhan, China. [4]Department of Cardiology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, China.

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关键词： acute myocardial infarction apoptosis CircSAMD4A H/R inflammatory response miR-138-5p

摘要：

Hypoxia/reoxygenation (H/R)-induced myocardial cell injury is the main cause of acute myocardial infarction (AMI). Many proofs show that circular RNA plays an important role in the development of AMI. The purpose of this study was to investigate the role of circSAMD4A in H/R-induced myocardial injury. The levels of circular SAMD4A (circSAMD4A) were detected in the heart tissues of AMI mice and H/R-induced H9C2 cells, and the circSAMD4A was suppressed in AMI mice and H/R-induced H9C2 cells to investigate its' function in AMI. The levels of circSAMD4A and miR-138-5p were detected by real-time quantitative PCR, and MTT assay was used to detect cell viability. TUNEL analysis and Annexin V-FITC were used to determine apoptosis. The expression of Bcl-2 and Bax proteins was detected by Western blot. IL-1β, TNF-α and IL-6 were detected by ELISA kits. The study found that the levels of circSAMD4A were up-regulated after H/R induction and inhibition of circSAMD4A expression would reduce the H/R-induced apoptosis and inflammation. MiR-138-5p was down-regulated in H/R-induced H9C2 cells. circSAMD4A was a targeted regulator of miR-138-5p. CircSAMD4A inhibited the expression of miR-138-5p to promote H/R-induced myocardial cell injury in vitro and vivo. In conclusion, CircSAMD4A can sponge miR-138-5p to promote H/R-induced apoptosis and inflammatory response.© 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

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出版当年[2019]版：

大类 | 2 区医学

小类 | 2 区医学：研究与实验 3 区细胞生物学

最新[2023]版：

大类 | 2 区

医学

小类 | 2 区医学：研究与实验 3 区细胞生物学

第一作者：

第一作者机构： [1]Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan, China.

通讯作者：

通讯机构： [1]Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan, China. [4]Department of Cardiology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, China. [*1]Department of Cardiology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, JiangXi, China. [*2]Department of Cardiology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, China.

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