Integration of OV6 expression and CD68+tumor-associated macrophages with clinical features better predicts the prognosis of patients with hepatocellular carcinoma
Background: Reliable prognostic indicators for accurately predicting postoperative outcomes in Hepatocellular carcinoma (HCC) patients are lacking. Although cancer stem-like cells (CSCs) and tumor-associated macrophages (TAMs) in tumor microenvironment are implicated in the occurrence and development of HCC, whether the combination of CSC biomarkers and TAM populations could achieve better performance in predicting the prognosis of patients with HCC has been rarely reported.Methods: A total of 306 HCC patients were randomly divided into the training and validation cohorts at a 1:1 ratio, and the expression of OV6 and CD68 was assessed using immunohistochemistry in HCC samples. The prognostic value of these biomarkers for post-surgical survival and recurrence were evaluated by the curve of receiver operating characteristic and multivariate Cox regression analyses. Results: The density of OV6+ CSCs was positively correlated with the infiltration of CD68+ TAMs in HCC. Both high OV6 expression and CD68+ TAM infiltration was closely associated with poor overall survival (OS) and progression-free survival (PFS) of HCC patients. Moreover, overexpression of OV6 and infiltration of CD68+ TAMs were identified as independent prognostic factors for OS and PFS after liver resection. The integration of OV6 and CD68 with tumor size and microvascular invasion exhibited highest C-index value for survival predictivity in HCC patients than any other biomarkers or clinical indicators alone.Conclusion: Incorporating intratumoral OV6 expression and CD68+ TAMs infiltration with established clinical indicators may serve as a promising prognostic signature for HCC, and could more accurately predict the clinical outcomes for HCC patients after liver resection.
基金：
National Natural Science Foundation of China [82173357, 81773154]; Natural Science Foundation of Shanghai [20ZR1449600, 22ZR1477900]; Shanghai Science and Technology Committee RisingStar Program [22QA1411600]; Pudong New Area Science and technology development fund special fund [PKJ2019-Y19]
第一作者机构：[1]Shanghai Pudong Gongli Hosp, Dept Urinary Surg, Shanghai 200135, Peoples R China[2]Shanghai Pudong Gongli Hosp, Shanghai Hlth Commiss Key Lab Artificial Intellige, Shanghai 200135, Peoples R China[3]Navy Med Univ, Eastern Hepatobiliary Surg Hosp, Dept Hepatobiliary Surg, Shanghai 200433, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Shanghai Pudong Gongli Hosp, Dept Urinary Surg, Shanghai 200135, Peoples R China[2]Shanghai Pudong Gongli Hosp, Shanghai Hlth Commiss Key Lab Artificial Intellige, Shanghai 200135, Peoples R China
推荐引用方式(GB/T 7714)：
Wang Ming-Da,Xiang Hao,Zhang Ling,et al.Integration of OV6 expression and CD68+tumor-associated macrophages with clinical features better predicts the prognosis of patients with hepatocellular carcinoma[J].TRANSLATIONAL ONCOLOGY.2022,25:doi:10.1016/j.tranon.2022.101509.
APA：
Wang, Ming-Da,Xiang, Hao,Zhang, Ling&Wang, Chao.(2022).Integration of OV6 expression and CD68+tumor-associated macrophages with clinical features better predicts the prognosis of patients with hepatocellular carcinoma.TRANSLATIONAL ONCOLOGY,25,
MLA：
Wang, Ming-Da,et al."Integration of OV6 expression and CD68+tumor-associated macrophages with clinical features better predicts the prognosis of patients with hepatocellular carcinoma".TRANSLATIONAL ONCOLOGY 25.(2022)
