机构：[1]Department of Orthopaedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai 200003, China[2]Department of Orthopaedics, Nantong Hospital Affiliated to Shanghai University (Nantong Sixth People’s Hospital), Nantong, Jiangsu 226011, China[3]Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China[4]Department of Orthopaedics, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China
Articular cartilage (AC) lesions are common but remain a great challenge for researchers and clinicians mainly due to their poor self-healing capability. Decellularized cartilage extracellular matrix (dCECM) derived from natural cartilage, and the incorporated growth factors have been used for potential chondrocyte induction and cartilage tissue regeneration. Moreover, infrapatellar fat pad adipose-derived stem cells (IPFP-ADSCs) have been proven to be an optimal source of seeded cells for AC lesion restoration. Therefore, the therapeutic role of dCECM-incorporated bioink with IPFP-ADSCs loaded in 3D (three-dimensional) scaffolds remain to be explored using an osteochondral defect model. In this work, we successfully extracted IPFP-ADSCs and isolated dCECMs, which were then mixed in a temperature-responsive hydrogel to form a 3D bioink. The 3D bioprinting scaffolds printed with dCECM successfully facilitated chondrogenic differentiation-related growth factor expression in IPFP-ADSCs. Most importantly, implantation of the 3D dCECMs with an IPFP-ADSC-loaded scaffold was capable of providing a favorable cell matrix for stem cell proliferation and promoted chondrogenic differentiation, thereby greatly enhancing successful cartilage repair in rabbit cartilage defects.
基金：
National Key Research and Development Project of China [2017YFC1103404]; Three -Year Action Plan for Talent Construction of Shanghai Changzheng Hospital-Pyramid Talent Project
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2021]版：
大类|1 区工程技术
小类|1 区工程：环境1 区工程：化工
最新[2023]版：
大类|1 区工程技术
小类|1 区工程：化工1 区工程：环境
JCR分区：
出版当年[2020]版：
Q1ENGINEERING, ENVIRONMENTALQ1ENGINEERING, CHEMICAL
第一作者机构：[1]Department of Orthopaedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai 200003, China[2]Department of Orthopaedics, Nantong Hospital Affiliated to Shanghai University (Nantong Sixth People’s Hospital), Nantong, Jiangsu 226011, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Orthopaedics, Shanghai Changzheng Hospital, Naval Medical University, Shanghai 200003, China[*1]Department of Orthopaedics, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai 200003, China.
推荐引用方式(GB/T 7714)：
Wu Jun,Han Yaguang,Fu Qiwei,et al.Application of tissue-derived bioink for articular cartilage lesion repair[J].CHEMICAL ENGINEERING JOURNAL.2022,450:doi:10.1016/j.cej.2022.138292.
APA：
Wu, Jun,Han, Yaguang,Fu, Qiwei,Hong, Yuping,Li, Lexiang...&Qian, Qirong.(2022).Application of tissue-derived bioink for articular cartilage lesion repair.CHEMICAL ENGINEERING JOURNAL,450,
MLA：
Wu, Jun,et al."Application of tissue-derived bioink for articular cartilage lesion repair".CHEMICAL ENGINEERING JOURNAL 450.(2022)
工程技术