机构：[1]Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing, China首都医科大学附属北京同仁医院临床科室变态反应科[2]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China首都医科大学附属北京同仁医院临床科室耳鼻咽喉-头颈外科[3]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China首都医科大学附属北京同仁医院研究所耳鼻咽喉科研究所[4]Beijing Laboratory of Allergic Diseases and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China首都医科大学附属北京同仁医院研究所耳鼻咽喉科研究所
Analysis of immune markers in nasal secretions has become crucial in the study of nasal diseases. We proposed the cotton piece method, a modified method, for the collection and processing of nasal secretions.The nasal secretions of 31 healthy control participants and 32 patients with nasal diseases were collected by the traditional sponge method and the cotton piece method, respectively. The concentrations of 14 cytokines and chemokines related to nasal diseases were detected.The properties of nasal secretions collected by the cotton piece method were more uniform than the sponge method. The concentration of IL-6 in the disease group collected by the cotton piece method was significantly higher than that in the control group (P = 0.002), and the cotton piece method could distinguish the positive detection rates of IL-1β (P = 0.031) and TNF-α (P = 0.001) between the control and disease groups. The levels of inflammatory mediators in nasal secretions could preliminarily distinguish different nasal diseases.The cotton piece method is a noninvasive and reliable method for collecting nasal secretions, which is beneficial for detecting local inflammatory and immune responses of the nasal mucosa.
基金：
This work was supported by grants from national key R&D program of China (2022YFC2504100), the program for the Changjiang scholars and innovative research team (IRT13082), CAMS innovation fund for medical sciences (2019-I2M-5-022), Beijing Municipal Science & Technology Commission (Z211100002921057), Capital’s funds for health improvement and research (2022-1-1091) and the Natural Science Foundation of China (81970849, 82071022, 82271141).
第一作者机构：[1]Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing, China[2]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China[3]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China[4]Beijing Laboratory of Allergic Diseases and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing, China[2]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China[3]Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China[4]Beijing Laboratory of Allergic Diseases and Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China[*1]Department of Otolaryngology Head and Neck surgery and department of Allergy, Beijing TongRen Hospital, Capital Medical University, No.1DongJiaoMinXiang, DongCheng District, Beijing 100730, P.R China[*2]Beijing Institute of Otolaryngology, No. 17 HouGouHuTong, DongCheng District, Beijing 100005, P.R. China
推荐引用方式(GB/T 7714)：
Xu Xu,Zhang Xu,Liu Dong,et al.Development of a method of nasal secretions sampling for local nasal inflammation studies[J].EXPERT REVIEW OF CLINICAL IMMUNOLOGY.2023,19(8):1013-1021.doi:10.1080/1744666X.2023.2228493.
APA：
Xu Xu,Zhang Xu,Liu Dong,Wang Kunpeng,Wang Yang...&Zhang Luo.(2023).Development of a method of nasal secretions sampling for local nasal inflammation studies.EXPERT REVIEW OF CLINICAL IMMUNOLOGY,19,(8)
MLA：
Xu Xu,et al."Development of a method of nasal secretions sampling for local nasal inflammation studies".EXPERT REVIEW OF CLINICAL IMMUNOLOGY 19..8(2023):1013-1021
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