机构：[1]Beijing Tongren Hospital, Capital Medical University, Beijing, China.首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.[3]Beijing Tongren Eye Center, Beijing, China.首都医科大学附属北京同仁医院首都医科大学附属同仁医院[4]Beijing Ophthalmology&Visual Sciences Key Lab, Beijing, China.首都医科大学附属同仁医院
Accumulated evidence has shown that microRNAs (miRNAs) are closely related to the pathogenesis and progression of senile cataracts. Here we investigate the effect of miR-29a-3p in cataractogenesis and determined the potential molecular mechanism involved.In this study, we constructed a selenite cataract model in rats and obtained the miRNAs related to cataracts by whole transcriptome sequencing. To investigate the effect and mechanism of miR-29a-3p on cataracts, we performed several in vivo and in vitro experiments, including CCK8 assay, flow cytometry, luciferase reporter assay, Edu assay, and western blot analysis.Sequencing data showed downregulation of miR-29a-3p in rats with selenite cataracts. Down-regulation of miR-29a-3p could promote lens epithelial cells (SRA01/04) proliferation and inhibit cell apoptosis, and miR-29a-3p silence could inhibit the development of cataracts. Additionally, CAND1 was a direct target gene for miR-29a-3p.These data demonstrate that miR-29a-3p inhibits apoptosis of lens epithelial cells by regulating CAND1, which may be a potential target for senile cataracts.
基金：
National Natural Science Foundation of
China [82271067].
第一作者机构：[1]Beijing Tongren Hospital, Capital Medical University, Beijing, China.
通讯作者：
通讯机构：[1]Beijing Tongren Hospital, Capital Medical University, Beijing, China.[2]The State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.[3]Beijing Tongren Eye Center, Beijing, China.[4]Beijing Ophthalmology&Visual Sciences Key Lab, Beijing, China.[*1]Beijing Tongren Hospital, Beijing 100730, China[*2]Nankai University, Tianjin 300071, China
推荐引用方式(GB/T 7714)：
Fang Rui,Li Hai-Long,Lv Ning-Xin,et al.Inhibition of miR-29a-3p Alleviates Apoptosis of Lens Epithelial Cells via Upregulation of CAND1[J].CURRENT EYE RESEARCH.2024,49(4):391-400.doi:10.1080/02713683.2023.2293457.
APA：
Fang Rui,Li Hai-Long,Lv Ning-Xin,Yue Pei-Lin,Jia Yu-Xuan...&Song Xu-Dong.(2024).Inhibition of miR-29a-3p Alleviates Apoptosis of Lens Epithelial Cells via Upregulation of CAND1.CURRENT EYE RESEARCH,49,(4)
MLA：
Fang Rui,et al."Inhibition of miR-29a-3p Alleviates Apoptosis of Lens Epithelial Cells via Upregulation of CAND1".CURRENT EYE RESEARCH 49..4(2024):391-400
