机构：[1]Department of Ophthalmology, Hongqi Hospital of Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang, China[2]Department of Genetics, Center for Genetics, National Research Institute for Family Planning, Beijing 100081, China
Age-related cataract (ARC) is the main cause of blindness in older individuals but its specific pathogenic mechanism is unclear. This study aimed to identify differentially expressed genes (DEGs) associated with ARC and to improve our understanding of the disease mechanism.Anterior capsule samples of the human lens were collected from ARC patients and healthy controls and used for RNA sequencing to detect DEGs. Identified DEGs underwent bioinformatics analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Subsequently, reverse transcription quantitative RT-qPCR was used to validate the different expression levels of selected genes.A total of 698 up-regulated DEGs and 414 down-regulated DEGs were identified in ARC patients compared with controls by transcriptome analysis. Through GO and KEGG bioinformatics analysis, the functions of significantly DEGs and their possible molecular mechanisms were determined. Sequencing results were verified by RT-qPCR as being accurate and reliable.This study identified several genes associated with ARC, which improves our knowledge of the disease mechanism.
基金：
This study was supported by the National Natural Science Foundation of
China (31601116), the Postdoctoral Science Foundation of China (2016
M601447), the Basic research projects of provincial institutions of higher
learning in Heilongjiang Province, China (No. 2019-KYYWFMY-0002) and the
Mudanjiang Medical College Graduate Innovation Research Project (No.
2019YJSCX-10MY).
第一作者机构：[1]Department of Ophthalmology, Hongqi Hospital of Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Wang Zhongying,Su Dongmei,Liu Shanhe,et al.RNA sequencing and bioinformatics analysis of human lens epithelial cells in age-related cataract.[J].BMC OPHTHALMOLOGY.2021,21(1):doi:10.1186/s12886-021-01915-5.
APA：
Wang Zhongying,Su Dongmei,Liu Shanhe,Zheng Guiqian,Zhang Gaobo...&Hu Shanshan.(2021).RNA sequencing and bioinformatics analysis of human lens epithelial cells in age-related cataract..BMC OPHTHALMOLOGY,21,(1)
MLA：
Wang Zhongying,et al."RNA sequencing and bioinformatics analysis of human lens epithelial cells in age-related cataract.".BMC OPHTHALMOLOGY 21..1(2021)
