机构：[1]Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.[2]National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science & Photomedicine, Shanghai Engineering Center for Precise Diagnosis & Treatment of Eye Diseases, Shanghai, PR China.[3]Xiangya School of Medicine, Central South University, Changsha, Hunan, PR China.[4]Department of Ophthalmology, Tong Ren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
Objective: To elucidate the role of the competitive endogenous RNA (ceRNA) network in immune infiltration of diabetic retinopathy (DR). Methods: We obtained differentially expressed (DE) circRNAs, miRNAs and mRNAs from the Gene Expression Omnibus database. Then, we identified immune infiltration by CIBERSORT and single-sample gene set enrichment analysis and discovered co-expression genes by weighted gene co-expression network analysis. Furthermore, STAT1-mediated Th1 differentiation was determined in DR cell models, DR patients and DR mouse models. Results: hsa_circ_0087100/hsa-miR-6743-5p/STAT1 was involved in immune infiltration of Th1 cells. Aberrant expression of the ceRNA network and STAT1-mediated Th1 differentiation was thus verified in vitro and in vivo. Conclusion: hsa_circ_0087100/hsa-miR-6743-5p/STAT1 may affect Th1 cell differentiation in DR.
基金：
This work was supported by the National Natural Science Foundation of China (nos. 81970811, 82372072) and the Domestic
Science and Technology Cooperation Project of Shanghai Municipal Science and Technology Commission (no. 21015800700).
第一作者机构：[1]Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.[2]National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science & Photomedicine, Shanghai Engineering Center for Precise Diagnosis & Treatment of Eye Diseases, Shanghai, PR China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Ophthalmology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.[2]National Clinical Research Center for Eye Diseases, Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai Engineering Center for Visual Science & Photomedicine, Shanghai Engineering Center for Precise Diagnosis & Treatment of Eye Diseases, Shanghai, PR China.
推荐引用方式(GB/T 7714)：
He Shuai,Lai Dongwei,Ma Chenggong,et al.hsa_circ_0087100/hsa-miR-6743-5p affects Th1 cell differentiation by regulating STAT1 in diabetic retinopathy[J].EPIGENOMICS.2024,16(7):427-444.doi:10.2217/epi-2023-0359.
APA：
He Shuai,Lai Dongwei,Ma Chenggong,Meng Chunren,Cai Chunyang...&Qiu Qinghua.(2024).hsa_circ_0087100/hsa-miR-6743-5p affects Th1 cell differentiation by regulating STAT1 in diabetic retinopathy.EPIGENOMICS,16,(7)
MLA：
He Shuai,et al."hsa_circ_0087100/hsa-miR-6743-5p affects Th1 cell differentiation by regulating STAT1 in diabetic retinopathy".EPIGENOMICS 16..7(2024):427-444
