Syringin, a natural bioactive compound extracted from Acanthopanax senticosus, has demonstrated potential therapeutic value in cancer treatment. However, its efficacy in treating renal cell carcinoma (RCC) remains unexplored. This study aims to investigate the therapeutic effects and underlying mechanisms of Syringin in RCC. In this study, network pharmacology, molecular docking validation, and bioinformatics were employed to predict the mechanisms by which Syringin affects RCC. In vitro experiments showed that Syringin inhibited RCC cell viability and reduced the IC50 of Sunitinib, enhancing its therapeutic effect. Syringin also inhibited RCC cell proliferation and migration and promoted apoptosis. The combination of Syringin and Sunitinib demonstrated an enhanced inhibitory effect. Western blot analysis confirmed that Syringin's anti-RCC effects are mediated through the EGFR/PI3K/Akt pathway. In conclusion, our findings indicate that Syringin exhibits inhibitory effects on RCC cells and enhances their sensitivity to Sunitinib, offering a novel approach to exploring treatment strategies for Sunitinib-resistant RCC.
基金：
National Natural Sci-ence Foundation of China [82172809]; Research Fund of Shanghai Tongren Hospital, Shanghai Jiaotong University School of Medicine [TRKYRC-xx202205, 2023DHYGJC-YBA04]
第一作者机构：[1]Shanghai Jiao Tong Univ, Sch Med, Dept Urol, Tongren Hosp, Shanghai 200336, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Shanghai Jiao Tong Univ, Sch Med, Dept Urol, Tongren Hosp, Shanghai 200336, Peoples R China[*1]1111 Xian Xia Rd, Shanghai, Peoples R China
推荐引用方式(GB/T 7714)：
Chen Zixuan,Cheng Sheng,Xu An,et al.Syringin as a novel therapeutic agent for renal cell carcinoma by targeting EGFR/PI3K/Akt pathway and enhancing sunitinib efficacy[J].JOURNAL OF FUNCTIONAL FOODS.2024,123:doi:10.1016/j.jff.2024.106556.
APA：
Chen, Zixuan,Cheng, Sheng,Xu, An,Han, Chengtao,Jia, Xing&Liu, Min.(2024).Syringin as a novel therapeutic agent for renal cell carcinoma by targeting EGFR/PI3K/Akt pathway and enhancing sunitinib efficacy.JOURNAL OF FUNCTIONAL FOODS,123,
MLA：
Chen, Zixuan,et al."Syringin as a novel therapeutic agent for renal cell carcinoma by targeting EGFR/PI3K/Akt pathway and enhancing sunitinib efficacy".JOURNAL OF FUNCTIONAL FOODS 123.(2024)
农林科学