Objective: To evaluate the transduction efficiency of a recombinant adenovirus carrying the gene for green fluorescent protein (Ad-GFP) into the primary cultures of fetal neural stem cells (NSCs) by the expression of GFP. Methods: The Ad-GFP was constructed by homologous recombination in bacteria with the AdEasy system; NSCs were isolated from rat fetal hippocampus and cultured as neurosphere suspensions. After infection with the recombinant Ad-GFP, NSCs were examined with a fluorescent microscopy and a f low cytometry for their expression of GFP.Results: After the viral infection, flow cytometry analysis revealed that the percentage of GFP-positive cells was as high as 97.05%. The infected NSCs sustained the GFP expression for above 4 weeks. After differentiated into astrocytes or neurons, they continued to express GFP efficiently. Conclusion: The have successfully constructed a viral vector Ad-GFP that can efficiently infect the primary NSCs. The reporter gene was showed fully and sustained expression in the infected cells as well as their differentiated progenies.
基金：
Project (No. 30672308) supported by the National Natural Science
Foundation of China
语种：
外文
被引次数：
WOS：
中科院(CAS)分区：
出版当年[2007]版：
无
最新[2023]版：
大类|3 区生物学
小类|3 区生物工程与应用微生物4 区生化与分子生物学4 区医学：研究与实验
JCR分区：
出版当年[2006]版：
无
最新[2023]版：
Q1BIOCHEMISTRY & MOLECULAR BIOLOGYQ1BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ1MEDICINE, RESEARCH & EXPERIMENTAL
第一作者机构：[1]Zhejiang Univ, Sch Med, Affiliated Hosp 1, Dept Otorhinolaryngol, Hangzhou 310003, Peoples R China
通讯作者：
推荐引用方式(GB/T 7714)：
Fu Yong,Wang Shen-qing,Liu Ying-peng,et al.Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein[J].JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B.2008,9(4):299-305.doi:10.1631/jzus.B0710446.
APA：
Fu, Yong,Wang, Shen-qing,Liu, Ying-peng,Wang, Guo-peng,Wang, Jian-ting&Gong, Shu-sheng.(2008).Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein.JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B,9,(4)
MLA：
Fu, Yong,et al."Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein".JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B 9..4(2008):299-305
