机构：[1]Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China临床科室耳鼻咽喉-头颈外科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Allergy and Cancer Center, Otorhinolaryngology Hospital of the First Affiliated Hospital of Sun Yat-sen University, No 58, Zhongshan 2nd Road, and Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, China中山大学附属第一医院
Objective: To evaluate the expression of mismatch repair gene PMS2 in human nasopharyngeal carcinoma (NPC) tissues and evaluate the effect of glycogen synthase kinase (GSK)-3 beta on PMS2 production in vivo and in vitro. Methods: The expression of PMS2 and inactivated phosphorylated GSK-3 beta(s9) was examined by immunohistochemical staining in 25 NPC tissues and the relation was determined by correlation analysis. The effect of GSK-3 beta transfection in CNE-2 cells on PMS2 production as well as cell apoptosis and chemosensitization were evaluated using small interference RNA (siRNA), immunoblotting and flow cytometric analysis in vitro. Results: The expression of inactivated phosphorylated GSK-3 beta(s9) was found to negative correlated with PMS2 in vivo. And transfected GSK-3 beta was found to be able to enhance PMS2 production, and increase cell apoptosis in CNE-2 cells in combination with cisplatin administration in vitro. Conclusion: Inactivation of GSK-3 beta might be important for NPC tumorgenesis through negatively regulating PMS2 production, and enhanced PMS2 production by GSK-3 beta is beneficial for understanding the NPC tumorgenesis and developing potential strategy for future therapy. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
基金：
National Nature and Science Grant, China [81072204, 81072224]
第一作者机构：[1]Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Fang Jugao,Lei Wenbin,Huang Xiaoming,et al.Expression of mismatch repair gene PMS2 in nasopharyngeal carcinoma and regulation by glycogen synthase kinase-3 beta in vivo and in vitro[J].AURIS NASUS LARYNX.2012,39(1):71-76.doi:10.1016/j.anl.2011.02.009.
APA：
Fang, Jugao,Lei, Wenbin,Huang, Xiaoming,Li, Pingdong,Chen, Xiaohong...&Li, Huabin.(2012).Expression of mismatch repair gene PMS2 in nasopharyngeal carcinoma and regulation by glycogen synthase kinase-3 beta in vivo and in vitro.AURIS NASUS LARYNX,39,(1)
MLA：
Fang, Jugao,et al."Expression of mismatch repair gene PMS2 in nasopharyngeal carcinoma and regulation by glycogen synthase kinase-3 beta in vivo and in vitro".AURIS NASUS LARYNX 39..1(2012):71-76
