Basic transcription factor 3 (BTF3), a transcription factor and modulator of apoptosis, is differentially expressed in carcinoma. To acquire further understanding of the involvement of BTF3 in carcinoma, the present study analyzed the expression of BTF3, as well as its role in cell function in nasopharyngeal carcinoma (NPC). BTF3 transcription rates in human NPC samples (n=46) and adjacent normal tissue samples (n=46) were analyzed using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry. BTF3-silencing in NPC cells was performed via specific small interfering RNA molecules. The function of BTF3 was analyzed by proliferation assays and colony forming assays using a Cellomic assay system. The positive expression rates of BTF3 were significantly increased in cancerous tissues compared with those in adjacent tissues (P<0.05). In addition, BTF3-silencing decreased cell proliferation and colony formation (P<0.01) in TCA-8113 and 5-8F cells. BTF3 is overexpressed in NPC, and its silencing is associated with decreased cell proliferation and colony formation, enhanced apoptosis and cell cycle regulation of TCA-8113 and 5-8F cells.
基金：
Beijing Health System High-level Health Technology Talents Training Project Funding [2015-3-022]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81670946]; Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [XMLX201507]; Capital's Funds for Health Improvement and Research [CFH2018-1-2052]; Beijing Municipal Administration of Hospitals Incubating Program [PX2017032]
第一作者机构：[1]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dongjiaomin Alley, Beijing 100730, Peoples R China
共同第一作者：
通讯作者：
通讯机构：[1]Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dongjiaomin Alley, Beijing 100730, Peoples R China[*1]Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, 1 Dongjiaomin Alley, Beijing 100730, P.R. China
推荐引用方式(GB/T 7714)：
Chen Ping,Zhong Qi,Li Zufei,et al.Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma[J].ONCOLOGY LETTERS.2019,17(1):789-796.doi:10.3892/ol.2018.9699.
APA：
Chen, Ping,Zhong, Qi,Li, Zufei,Zhang, Yang&Huang, Zhigang.(2019).Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma.ONCOLOGY LETTERS,17,(1)
MLA：
Chen, Ping,et al."Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma".ONCOLOGY LETTERS 17..1(2019):789-796
