机构：[1]Department of Oral & Maxillofacial-Head Neck Oncology, Ninth People's Hospital, College of Stomatology, Shanghai JiaoTong University, School of Medicine, Shanghai, 200011, China[2]Department of Orthopedics, Tong Ren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336, China
Objective: To explore the inhibitory effects of zoledronate (ZOL) on adipose-derived stem cells (ADSCs) into osteoblasts for repairing jaw necrosis. Methods: ADSCs were induced to differentiate into osteoblasts. The differentiation characteristics of osteoblasts was observed under inverted microscope by alizarin red staining. The transwell assay was performed to evaluate the migration of ADSCs co-cultured with osteoblasts and divided into ZOL group treated with ZOL and N-ZOL group without ZOL treatment. The differentiation and proliferation characteristics of ADSCs differentiated osteoblasts were observed respectively. The expression of CTSK (Cathepsin K) and FGFR3 (Fibroblast growth factor receptor 3) in osteoblasts were analyzed by immunofluorescence and western blot. Results: The differentiation degree and proliferation of ADSCs to osteoblasts in N-ZOL group were both higher than those in ZOL group. The migratory cell number in ADSCs differentiation in ZOL group was higher than that of N-ZOL group. The protein expression of CTSK and FGFR3 in ADSCs differentiated to osteoblasts in ZOL group was higher than that in N-ZOL group. Conclusion: The differentiation of ADSCs into osteoblasts is significantly inhibited by ZOL. Due to this reason, it may be difficult to achieve good results by ZOL induced ADSCs into osteoblasts in repairing jaw necrosis.
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|4 区生物
小类|4 区生化研究方法4 区生化与分子生物学4 区生物工程与应用微生物4 区细胞生物学
最新[2023]版：
大类|3 区生物学
小类|2 区生化研究方法2 区生物工程与应用微生物3 区生化与分子生物学3 区细胞生物学
JCR分区：
出版当年[2018]版：
Q2BIOCHEMICAL RESEARCH METHODSQ2BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ3BIOCHEMISTRY & MOLECULAR BIOLOGYQ3CELL BIOLOGY
最新[2023]版：
Q3BIOCHEMISTRY & MOLECULAR BIOLOGYQ3BIOCHEMICAL RESEARCH METHODSQ3BIOTECHNOLOGY & APPLIED MICROBIOLOGYQ4CELL BIOLOGY
第一作者机构：[1]Department of Oral & Maxillofacial-Head Neck Oncology, Ninth People's Hospital, College of Stomatology, Shanghai JiaoTong University, School of Medicine, Shanghai, 200011, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Orthopedics, Tong Ren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200336, China[*1]Department of Orthopedics, Tong Ren Hospital, Shanghai Jiao Tong University School of Medicine, No. 1111 XianXia Road, Shanghai, 200336, China.
推荐引用方式(GB/T 7714)：
Qu Xingzhou,Wang Zhen,Wu Kailiu,et al.Zoledronate inhibits the differentiation potential of adipose-derived stem cells into osteoblasts in repairing jaw necrosis[J].MOLECULAR AND CELLULAR PROBES.2020,51:doi:10.1016/j.mcp.2020.101525.
APA：
Qu, Xingzhou,Wang, Zhen,Wu, Kailiu,Wang, Yang&Shan, Liancheng.(2020).Zoledronate inhibits the differentiation potential of adipose-derived stem cells into osteoblasts in repairing jaw necrosis.MOLECULAR AND CELLULAR PROBES,51,
MLA：
Qu, Xingzhou,et al."Zoledronate inhibits the differentiation potential of adipose-derived stem cells into osteoblasts in repairing jaw necrosis".MOLECULAR AND CELLULAR PROBES 51.(2020)
