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Particulate Matter 2.5 Causes Deficiency in Barrier Integrity in Human Nasal Epithelial Cells

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机构: [1]Capital Med Univ, Beijing TongRen Hosp, Dept Otolaryngol Head & Neck Surg, 1 DongJiaoMinXiang St, Beijing 100730, Peoples R China [2]Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, Beijing, Peoples R China [3]Univ Zurich, Swiss Inst Allergy & Asthma Res SIAF, Davos, Switzerland [4]Christine Kuhne Ctr Allergy Res & Educ, Davos, Switzerland
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关键词: Epithelial cells particulate matters nasal mucosa rhinitis asthma cytokines

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Purpose: The effect of air pollution-related particulate matter (PM) on epithelial barrier function and tight junction (TJ) expression in human nasal mucosa has not been studied to date. This study therefore aimed to assess the direct impact of PM with an aerodynamic diameter less than 2.5 mu m (PM2.5) on the barrier function and TJ molecular expression of human nasal epithelial cells. Methods: Air-liquid interface cultures were established with epithelial cells derived from noninflammatory nasal mucosal tissue collected from patients undergoing paranasal sinus surgery. Confluent cultures were exposed to 50 or 100 mu g/mL PM2.5 for up to 72 hours, and assessed for 1) epithelial barrier integrity as measured by transepithelial resistance (TER) and permeability of fluorescein isothiocyanate (FITC) 4 kDa; 2) expression of TJs using real-time quantitative polymerase chain reaction and immunofluorescence staining, and 3) proinflammatory cytokines by luminometric bead array or enzyme-linked immunosorbent assay. Results: Compared to control medium, 50 and/or 100 mu g/mL PM2.5-treatment 1) significantly decreased TER and increased FITC permeability, which could not be restored by budesonide pretreatment; 2) significantly decreased the expression of claudin-1 messenger RNA, claudin-1, occludin and ZO-1 protein; and 3) significantly increased production of the cytokines interleukin-8, TIMP metallopeptidase inhibitor 1 and thymic stromal lymphopoietin. Conclusions: Exposure to PM2.5 may lead to loss of barrier function in human nasal epithelium through decreased expression of TJ proteins and increased release of proinflammatory cytokines. These results suggest an important mechanism of susceptibility to rhinitis and rhinosinusitis in highly PM2.5-polluted areas.

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出版当年[2019]版:
大类 | 2 区 医学
小类 | 3 区 过敏 3 区 免疫学
最新[2023]版:
大类 | 2 区 医学
小类 | 2 区 免疫学 3 区 过敏
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出版当年[2018]版:
Q1 ALLERGY Q1 IMMUNOLOGY
最新[2023]版:
Q2 ALLERGY Q2 IMMUNOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2018版] 出版当年五年平均 出版前一年[2017版] 出版后一年[2019版]

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第一作者机构: [1]Capital Med Univ, Beijing TongRen Hosp, Dept Otolaryngol Head & Neck Surg, 1 DongJiaoMinXiang St, Beijing 100730, Peoples R China [2]Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, Beijing, Peoples R China
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通讯机构: [1]Capital Med Univ, Beijing TongRen Hosp, Dept Otolaryngol Head & Neck Surg, 1 DongJiaoMinXiang St, Beijing 100730, Peoples R China [2]Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, Beijing, Peoples R China [*1]Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, No. 1, DongJiaoMinXiang Street, DongCheng District, Beijing 100730, China. [*2]Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, No. 1, DongJiaoMinXiang Street, DongCheng District, Beijing 100730, China.
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