Purpose: The effect of air pollution-related particulate matter (PM) on epithelial barrier function and tight junction (TJ) expression in human nasal mucosa has not been studied to date. This study therefore aimed to assess the direct impact of PM with an aerodynamic diameter less than 2.5 mu m (PM2.5) on the barrier function and TJ molecular expression of human nasal epithelial cells. Methods: Air-liquid interface cultures were established with epithelial cells derived from noninflammatory nasal mucosal tissue collected from patients undergoing paranasal sinus surgery. Confluent cultures were exposed to 50 or 100 mu g/mL PM2.5 for up to 72 hours, and assessed for 1) epithelial barrier integrity as measured by transepithelial resistance (TER) and permeability of fluorescein isothiocyanate (FITC) 4 kDa; 2) expression of TJs using real-time quantitative polymerase chain reaction and immunofluorescence staining, and 3) proinflammatory cytokines by luminometric bead array or enzyme-linked immunosorbent assay. Results: Compared to control medium, 50 and/or 100 mu g/mL PM2.5-treatment 1) significantly decreased TER and increased FITC permeability, which could not be restored by budesonide pretreatment; 2) significantly decreased the expression of claudin-1 messenger RNA, claudin-1, occludin and ZO-1 protein; and 3) significantly increased production of the cytokines interleukin-8, TIMP metallopeptidase inhibitor 1 and thymic stromal lymphopoietin. Conclusions: Exposure to PM2.5 may lead to loss of barrier function in human nasal epithelium through decreased expression of TJ proteins and increased release of proinflammatory cytokines. These results suggest an important mechanism of susceptibility to rhinitis and rhinosinusitis in highly PM2.5-polluted areas.
基金:
national key R&D program of China [2016YFC20160905200]; program for the Changjiang scholars and innovative research teamProgram for Changjiang Scholars & Innovative Research Team in University (PCSIRT) [IRT13082]; national natural science foundation of ChinaNational Natural Science Foundation of China (NSFC) [81500771, 81470678, 81870698, 81420108009, 81630023]; Beijing municipal administration of hospitals' mission plan [SML20150203]; Beijing advanced innovation center for food nutrition and human health (Beijing Technology and Business University) [20181045]; Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support [XMLX201816]; Capital Health Development Foundation [2016-1-2052]
第一作者机构:[1]Capital Med Univ, Beijing TongRen Hosp, Dept Otolaryngol Head & Neck Surg, 1 DongJiaoMinXiang St, Beijing 100730, Peoples R China[2]Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, Beijing, Peoples R China
共同第一作者:
通讯作者:
通讯机构:[1]Capital Med Univ, Beijing TongRen Hosp, Dept Otolaryngol Head & Neck Surg, 1 DongJiaoMinXiang St, Beijing 100730, Peoples R China[2]Beijing Inst Otolaryngol, Beijing Key Lab Nasal Dis, Beijing, Peoples R China[*1]Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, No. 1, DongJiaoMinXiang Street, DongCheng District, Beijing 100730, China.[*2]Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, No. 1, DongJiaoMinXiang Street, DongCheng District, Beijing 100730, China.
推荐引用方式(GB/T 7714):
Xian Mu,Ma Siyuan,Wang Kuiji,et al.Particulate Matter 2.5 Causes Deficiency in Barrier Integrity in Human Nasal Epithelial Cells[J].ALLERGY ASTHMA & IMMUNOLOGY RESEARCH.2020,12(1):56-71.doi:10.4168/aair.2020.12.1.56.
APA:
Xian, Mu,Ma, Siyuan,Wang, Kuiji,Lou, Hongfei,Wang, Yang...&Akdis, Cezmi A..(2020).Particulate Matter 2.5 Causes Deficiency in Barrier Integrity in Human Nasal Epithelial Cells.ALLERGY ASTHMA & IMMUNOLOGY RESEARCH,12,(1)
MLA:
Xian, Mu,et al."Particulate Matter 2.5 Causes Deficiency in Barrier Integrity in Human Nasal Epithelial Cells".ALLERGY ASTHMA & IMMUNOLOGY RESEARCH 12..1(2020):56-71