机构:[1]School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China[2]Jiangsu Institute of Nuclear Medicine, Wuxi, China[3]Wuxi Children’s Hospital, Wuxi People’s Hospital affiliated to Nanjing Medical University, Wuxi, China[4]Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69-2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 +/- 15.15 ng/mL in 45 healthy volunteers, 31.08 +/- 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 +/- 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 +/- 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN. (C) 2019 The Author(s) Published by S. Karger AG, Basel
基金:
Clinical Medical Science fund of Jiangsu Province (No.
BL2014022), Research Project of Jiangsu Provincial Commission of Health and Family
Planning (No. H201637), Science and Technology Commission of Shanghai Municipality (No.
15ZR1437900), Wuxi Key Medical Talents (No. zdrc006), Jiangsu Province Science and
Education Project (No. CXTDB2017016), Medical and Public Health Project of Wuxi Sci-Tech
Development Fund (No. WX18IIAN027), Medical and Public Health Project of Wuxi Sci-Tech
Development Fund (No. WX18IIAN647), and Wuxi Young Medical Talents (No. Q201705).
第一作者机构:[1]School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China[2]Jiangsu Institute of Nuclear Medicine, Wuxi, China
共同第一作者:
通讯作者:
通讯机构:[3]Wuxi Children’s Hospital, Wuxi People’s Hospital affiliated to Nanjing Medical University, Wuxi, China[4]Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China[*1]No. 1111, Xianxia Road Shanghai 200336 (China)[*2]299 Qingyang Road Wuxi 214023 (China)
推荐引用方式(GB/T 7714):
Huang Biao,Zhang Yi,Wang Liang,et al.Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy[J].KIDNEY & BLOOD PRESSURE RESEARCH.2019,44(4):848-857.doi:10.1159/000500456.
APA:
Huang, Biao,Zhang, Yi,Wang, Liang,Xu, Wenwei,Zhang, Jue...&Hu, Zhigang.(2019).Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy.KIDNEY & BLOOD PRESSURE RESEARCH,44,(4)
MLA:
Huang, Biao,et al."Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy".KIDNEY & BLOOD PRESSURE RESEARCH 44..4(2019):848-857