高级检索
当前位置: 首页 > 详情页

Phospholipase A2 Receptor Antibody IgG4 Subclass Improves Sensitivity and Specificity in the Diagnosis of Idiopathic Membranous Nephropathy

文献详情

资源类型:
WOS体系:
Pubmed体系:

收录情况: ◇ SCIE

机构: [1]School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China [2]Jiangsu Institute of Nuclear Medicine, Wuxi, China [3]Wuxi Children’s Hospital, Wuxi People’s Hospital affiliated to Nanjing Medical University, Wuxi, China [4]Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
出处:
ISSN:

关键词: Phospholipase A2 receptor antibody IgG4 Idiopathic membranous nephropathy Membranous nephropathy Time-resolved fluoroimmunoassay

摘要:
Aims: The aim of this study was to develop a new method for detecting anti-phospholipase A2 receptor-IgG4 to improve the sensitivity and specificity in the diagnosis of idiopathic membranous nephropathy (IMN). Methods: A highly sensitive quantitative assay was developed for the detection of serum anti-phospholipase A2 receptor-IgG4 with europium chelation by time-resolved fluoroimmunoassay (TRFIA), and a mouse anti-human IgG4 tracer was prepared using europium chelation for detection. The specificity and sensitivity of anti-phospholipase A2 receptor-IgG4 in the diagnosis of IMN were further assessed in patients with different kidney diseases. Results: The detection limit of anti-PLA2R-IgG4 was 0.69 ng/mL. The measurement range of anti-PLA2R-IgG4 TRFIA was 0.69-2,500 ng/mL. Mean serum anti-PLA2R-IgG4 was 21.27 +/- 15.15 ng/mL in 45 healthy volunteers, 31.08 +/- 18.17 ng/mL in 29 IgA nephropathy patients, 49.10 +/- 34.32 ng/mL in 8 lupus nephropathy patients, and 10,324.11 +/- 17,030.40 ng/mL in 30 IMN patients. The anti-PLA2R-IgG4 cutoff concentration was >161.2 ng/mL with the sensitivity of 90.0% and specificity of 100% in the diagnosis of IMN. However, the cutoff for other kidney diseases was lower than 161.2 ng/mL. Conclusion: The serum anti-phospholipase A2 receptor IgG4 detected with the method developed in this study has higher sensitivity and higher specificity than total IgG in the diagnosis of IMN. (C) 2019 The Author(s) Published by S. Karger AG, Basel

基金:
语种:
被引次数:
WOS:
PubmedID:
中科院(CAS)分区:
出版当年[2018]版:
大类 | 3 区 医学
小类 | 3 区 外周血管病 3 区 生理学 3 区 泌尿学与肾脏学
最新[2023]版:
大类 | 4 区 医学
小类 | 3 区 生理学 4 区 外周血管病 4 区 泌尿学与肾脏学
JCR分区:
出版当年[2017]版:
Q2 UROLOGY & NEPHROLOGY Q2 PERIPHERAL VASCULAR DISEASE Q2 PHYSIOLOGY
最新[2023]版:
Q2 PERIPHERAL VASCULAR DISEASE Q2 UROLOGY & NEPHROLOGY Q3 PHYSIOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2017版] 出版当年五年平均 出版前一年[2016版] 出版后一年[2018版]

第一作者:
第一作者机构: [1]School of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, China [2]Jiangsu Institute of Nuclear Medicine, Wuxi, China
共同第一作者:
通讯作者:
通讯机构: [3]Wuxi Children’s Hospital, Wuxi People’s Hospital affiliated to Nanjing Medical University, Wuxi, China [4]Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China [*1]No. 1111, Xianxia Road Shanghai 200336 (China) [*2]299 Qingyang Road Wuxi 214023 (China)
推荐引用方式(GB/T 7714):
APA:
MLA:

资源点击量:21169 今日访问量:0 总访问量:1219 更新日期:2025-01-01 建议使用谷歌、火狐浏览器 常见问题

版权所有©2020 首都医科大学附属北京同仁医院 技术支持:重庆聚合科技有限公司 地址:北京市东城区东交民巷1号(100730)