机构：[1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Lab, Beijing 100730, China首都医科大学附属北京同仁医院首都医科大学附属同仁医院[2]Departments of Ophthalmology and Pathology, New York Eye and Ear Infirmary of Mount Sinai, 310 E.14th St., NY 10003, USA[3]Department of Ophthalmology, Medical Faculty Mannheim of the Ruprecht-Karls-University, Heidelberg 67117, Germany
AIM: To examine the genetic profile of primary uveal melanoma (UM) as compared to UM in immune escape. METHODS: Dendritic cells (DC) loaded with lysates of UM cells of high metastatic potential were used to stimulate cytotoxic T-lymphocytes (CTLs). When CTLs co-cultured with the UM cells, most UM cells could be eliminated. Survival UM cells grew slowly and were considered to be survival variants and examined by a microarray analysis. These differential genes were analyzed further with Venn Diagrams and functions related to immune escape. We additionally examined transcriptional changes of manually selected survival variants of UM cells and of clinical UM samples by quantitativereal-timepolymerasechainreaction(qRT-PCR), and analyzed the correlation of these expressions and patients' survival. RESULTS: Gene expression analyses revealed a marked up -regulation of SLAMF7 and CCL22 and a significant down -regulation of KRT10 and FXYD3 and ABCC2. The expression of these genes in the relapsed UM was significantly greater than those in primary UM. UM patients with overexpression of these genes had a shorter survival period as compared with those of their underexpression. CONCLUSION: Gene expression, in particular of SLAMF7, CCL22, KRT10, FXYD3 and ABCC2 differed between primary UM cells and survival variants of UM cells.
基金：
National Natural Science
Foundation of China (No.81570891; No.81272981); the Beijing
Municipal Administration of Hospitals' Ascent Plan (No.
DFL20150201); Science & Technology Project of Beijing
Municipal Science & Technology Commission (No.
Z151100001615052); Beijing Municipal Administration of
Hospitals Clinical Medicine Development of Special Funding
Support (No.ZYLX201307); Beijing Natural Science
Foundation (No.7151003); Advanced Health Care
Professionals Development Project of Beijing Municipal
Health Bureau (No.2014-2-003).
第一作者机构：[1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Lab, Beijing 100730, China
通讯作者：
通讯机构：[1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Lab, Beijing 100730, China[*1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology and Visual Science Key Lab, No.1 Dong Jiao Min Xiang, Dong Cheng District, Beijing 100730, China
推荐引用方式(GB/T 7714)：
Hou Fang,Huang Qi-Ming,Hu Dan-Ning,et al.Immune oppression array elucidating immune escape and survival mechanisms in uveal melanoma[J].INTERNATIONAL JOURNAL OF OPHTHALMOLOGY.2016,9(12):1701-1712.doi:10.18240/ijo.2016.12.01.
APA：
Hou, Fang,Huang, Qi-Ming,Hu, Dan-Ning,Jonas, Jost B.&Wei, Wen-Bin.(2016).Immune oppression array elucidating immune escape and survival mechanisms in uveal melanoma.INTERNATIONAL JOURNAL OF OPHTHALMOLOGY,9,(12)
MLA：
Hou, Fang,et al."Immune oppression array elucidating immune escape and survival mechanisms in uveal melanoma".INTERNATIONAL JOURNAL OF OPHTHALMOLOGY 9..12(2016):1701-1712
