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Biomechanical alterations of dendritic cells by co-culturing with K562 CML cells and their potential role in immune escape

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资源类型：
WOS体系：
Pubmed体系：

收录情况： ◇ SCIE

作者：
Xu, Xiaofeng[1,2] * ; Zeng, Zhu[2,3,4,5] * ; Yao, Weijuan[2] ; Wang, Xianwei[2] ; Sun, Dagong[6] ; Ka, Weibo[6] ; Zhang, Yingyu[2] ; Wang, Xifu[7] ; Chen, Xiaopeng[8] ; Zha, Yi[9] ; Sun, Li[9] ; Xieg, Lide[8] ; Wen, Zongyao[2,*1] ; Chien, Shu[5] ;
机构： [1]Capital Med Univ, Beijing Tongren Hosp, Dept Lab Med, Beijing 100730, Peoples R China [2]Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Hemorheol Ctr, Beijing 100191, Peoples R China [3]Guiyang Med Coll, Guiyang 550004, Guizhou, Peoples R China [4]Univ Calif San Diego, Dept Bioengn, La Jolla, CA 92093 USA [5]Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA [6]Peking Univ, Hlth Sci Ctr, Dept Med Phys, Beijing 100191, Peoples R China [7]Peking Univ, Shougang Hosp, Dept Cardiol, Beijing 100144, Peoples R China [8]Chengde Med Coll, Chengde 067000, Peoples R China [9]Beijing Red Cross Blood Ctr, Beijing 100191, Peoples R China
出处：
DOI： 10.1016/j.jbiomech.2010.04.028
ISSN：

关键词： Dendritic cells Biomechanics Immunology Proteomics K562

摘要：
Dendritic cells (DCs), which are potent antigen presenting cells (APCs), are utilized to deliver the signals essential for the initiation of immune responses. In this study, we used an interdisciplinary approach to characterize the effect of K562 cells, a human chronic myeloid leukemia (CML) cell line, on the biomechanical characteristics and immune functions of DCs. When co-cultured with K562 cells, the biomechanical and immunological characteristics of immature DCs (imDCs) and mature DCs (mDCs) were severely impaired compared with controls. The changes include increased membrane viscoelasticity, reorganized cytoskeleton (F-actin), suppressed capability of antigen uptake, transen-dothelium migration, and activation of naive T cells. In exploring the mechanisms of these changes, we identified several genes and proteins by microarray analysis and 2D gel electrophoresis. Changes were found in the cytoskeleton-related genes and proteins (such as cofilin1 and profilin1) and matrix-related genes and proteins (such as TIMP1 and MMP9). These findings provide a molecular basis for the biomechanical and immunological changes of DCs in response to K562 and may help to elucidate the mechanism for tumor immune escape. (C) 2010 Elsevier Ltd. All rights reserved.

基金：
National Natural Science Foundations of ChinaNational Natural Science Foundation of China (NSFC) [10572007, 30770532]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2009]版：
大类 | 3 区 生物
小类 | 2 区 工程：生物医学 3 区 生物物理
最新[2023]版：
大类 | 3 区 医学
小类 | 2 区 生物物理 3 区 工程：生物医学
JCR分区：
出版当年[2008]版：
Q1 ENGINEERING, BIOMEDICAL Q2 BIOPHYSICS
最新[2023]版：
Q3 ENGINEERING, BIOMEDICAL Q3 BIOPHYSICS

影响因子： 最新[2023版] 最新五年平均 出版当年[2008版] 出版当年五年平均 出版前一年[2007版] 出版后一年[2009版]

第一作者：
第一作者机构： [1]Capital Med Univ, Beijing Tongren Hosp, Dept Lab Med, Beijing 100730, Peoples R China [2]Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Hemorheol Ctr, Beijing 100191, Peoples R China
共同第一作者：
通讯作者：
通讯机构： [2]Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Hemorheol Ctr, Beijing 100191, Peoples R China [*1]Peking Univ, Dept Biophys, Hlth Sci Ctr, Beijing 100191, Peoples R China
推荐引用方式(GB/T 7714)：
Xu Xiaofeng,Zeng Zhu,Yao Weijuan,et al.Biomechanical alterations of dendritic cells by co-culturing with K562 CML cells and their potential role in immune escape[J].JOURNAL OF BIOMECHANICS.2010,43(12):2339-2347.doi:10.1016/j.jbiomech.2010.04.028.
APA：
Xu, Xiaofeng,Zeng, Zhu,Yao, Weijuan,Wang, Xianwei,Sun, Dagong...&Chien, Shu.(2010).Biomechanical alterations of dendritic cells by co-culturing with K562 CML cells and their potential role in immune escape.JOURNAL OF BIOMECHANICS,43,(12)
MLA：
Xu, Xiaofeng,et al."Biomechanical alterations of dendritic cells by co-culturing with K562 CML cells and their potential role in immune escape".JOURNAL OF BIOMECHANICS 43..12(2010):2339-2347

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