Purpose To investigate the differentially expressed proteins (DEP) between high myopia and moderate myopia on the anterior corneal stroma. Methods Tandem mass tag (TMT) quantitative proteomics was utilized to reveal proteins. DEPs were screened by the multiple change of more than 1.2 times or less than 0.83 and the P value < 0.05. The DEPs were functional annotated by Gene Ontology (GO) terms. Proteins and protein interaction (PPI) networks were conducted with String online tool. Parallel reaction monitoring (PRM) data processing was used to verify the TMT proteomics results. Results There are 36 DEPs between high myopia and moderate myopia on the anterior corneal stroma, of which 11 proteins are upregulated, 25 proteins are downregulated. The GO analysis demonstrated keratinocyte migration and structural constituent of cytoskeleton that are significantly changed with most of the proteins decreased in high myopic corneas. Keratin 16 (KRT16) and erythrocyte membrane protein band 4.1-like protein 4B are the only two proteins involved in both functions. The PPI analysis showed keratin type II cytoskeletal 6A (KRT6A) and KRT16 that have strong connections. Immunoglobulin lambda variable 8-61(IGLV8-61) and nicotinamide phosphoribosyl transferase (NAMPT) have consistent results with the TMT. Conclusions The high myopic corneas have 36 DEPs compared to the moderate myopic corneas on the anterior corneal stroma. Keratinocyte migrations and structural constituent of cytoskeleton are weakened in high myopic corneas, which may partly account for the lower corneal biomechanics in high myopic eyes. The lower expressed KRT16 plays important roles in high myopic corneas.
基金:
National Natural Science Foundation of China [81900828]; Beijing Municipal Commission of Science and Technology Capital Clinical Diagnosis and Treatment Technology Research and Transformation Application [Z201100005520043]
第一作者机构:[1]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, 1 Dongjiaomin Lane, Beijing 100730, Peoples R China
通讯作者:
推荐引用方式(GB/T 7714):
Wu Wenjing,Xu Yushan,Zhang Fengju.Comparisons of the protein expressions between high myopia and moderate myopia on the anterior corneal stroma in human[J].GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY.2023,261(12):3549-3558.doi:10.1007/s00417-023-06158-2.
APA:
Wu, Wenjing,Xu, Yushan&Zhang, Fengju.(2023).Comparisons of the protein expressions between high myopia and moderate myopia on the anterior corneal stroma in human.GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY,261,(12)
MLA:
Wu, Wenjing,et al."Comparisons of the protein expressions between high myopia and moderate myopia on the anterior corneal stroma in human".GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY 261..12(2023):3549-3558