机构：[1]Division of Cardiology, TongRen Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China[2]School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, China
Laser radiation is an efficient approach for rapid improvement of industrial microbial phenotypes. To improve ethanol tolerance in Saccharomyces cerevisiae strains, a 266 nm laser radiation with the use of repetitive cultivation was explored in this work. After irradiated by 266 nm laser radiation and repetitive cultivation, a genetically stable SM4 strain was obtained. The SM4 strain could grow on YPD plate with extra 15% (v/v) ethanol. Moreover, the ethanol production performance of SM4 strain was 29.25% more than that of the wild type strain when they were cultivated in 5% (v/v) ethanol fermentation medium for 72 h. The DNA mutation was the possible characters for the phenotype of SM4 strain. Overall, the 266 nm laser radiation and repetitive cultivation approach might be a novel and useful for breeding fermentation microorganisms. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
基金：
973 project of the
Ministry of Science and Technology (Grant No. 2010CB912604), the
International S&T Cooperation Program of China, (Grant No.
0102011DFA32980), the National Natural Science Foundation of
China (Grant No. 81271694), the Science and Technology Commission of Shanghai Municipality (Grant No. 11411951500 and
12nm0502200), and the Fundamental Research Funds for the
Central Universities.
第一作者机构：[1]Division of Cardiology, TongRen Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhang Min,Zhu Rongrong,Zhang Minfeng,et al.Creation of an ethanol-tolerant Saccharomyces cerevisiae strain by 266 nm laser radiation and repetitive cultivation[J].JOURNAL OF BIOSCIENCE AND BIOENGINEERING.2014,118(5):508-513.doi:10.1016/j.jbiosc.2014.04.016.
APA：
Zhang, Min,Zhu, Rongrong,Zhang, Minfeng&Wang, Shilong.(2014).Creation of an ethanol-tolerant Saccharomyces cerevisiae strain by 266 nm laser radiation and repetitive cultivation.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,118,(5)
MLA：
Zhang, Min,et al."Creation of an ethanol-tolerant Saccharomyces cerevisiae strain by 266 nm laser radiation and repetitive cultivation".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 118..5(2014):508-513
