机构：[1]Departments of Anatomy, Pediatrics and Environmental Medicine, Quanzhou Medical College, Quanzhou, Fujian, China[2]Department of Otolaryngology Head and Neck Surgery and Department of Allergy, Beijing TongRen Hospital, Affiliated with the Capital University of Medical Science, Beijing, China临床科室耳鼻咽喉-头颈外科变态反应科首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Department of Microbiology, Faculty of Medicine, Oita University, Oita, Japan[4]Second Department of Biochemistry, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan
Energy-metabolism oscillations (EMO) are ultradian biological rhythms observed in in aerobic chemostat cultures of Saccharomyces cerevisiae. EMO regulates energy metabolism such as glucose, carbohydrate storage, O-2 uptake, and CO2 production. PSK1 is a nutrient responsive protein kinase involved in regulation of glucose metabolism, sensory response to light, oxygen, and redox state. The aim of this investigation was to assess the function of PSK1 in regulation of EMO. The mRNA levels of PSK1 fluctuated in concert with EMO, and deletion of PSK1 resulted in unstable EMO with disappearance of the fluctuations and reduced amplitude, compared with the wild type. Furthermore, the mutant PSK1 Delta showed downregulation of the synthesis and breakdown of glycogen with resultant decrease in glucose concentrations. The redox state represented by NADH also decreased in PSK1 Delta compared with the wild type. These data suggest that PSK1 plays an important role in the regulation of energy metabolism and stabilizes ultradian biological rhythms. These results enhance our understanding of the mechanisms of biorhythms in the budding yeast.
基金：
Program for Tongjiang Scholars from
Quanzhou City Government; Grant-in-Aid for
Scientific Research from the Yamanashi
University; Grant from the national key
institution development supportive program of
Quanzhou Medical College (2013-2015)
第一作者机构：[1]Departments of Anatomy, Pediatrics and Environmental Medicine, Quanzhou Medical College, Quanzhou, Fujian, China
通讯作者：
通讯机构：[1]Departments of Anatomy, Pediatrics and Environmental Medicine, Quanzhou Medical College, Quanzhou, Fujian, China[4]Second Department of Biochemistry, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi, Japan[*1]Department of Environment Medicine, Quanzhou Medical College, Anji Rd.2, Luojiang District, Quanzhou 362011, Fujian, China.
推荐引用方式(GB/T 7714)：
Xu Xianyan,Huang Meixian,Ouyang Yuhui,et al.PSK1 coordinates glucose metabolism and utilization and regulates energy-metabolism oscillation in Saccharomyces cerevisiae[J].YEAST.2020,37(3):261-268.doi:10.1002/yea.3458.
APA：
Xu, Xianyan,Huang, Meixian,Ouyang, Yuhui,Iha, Hidekatsu&Xu, Zhaojun.(2020).PSK1 coordinates glucose metabolism and utilization and regulates energy-metabolism oscillation in Saccharomyces cerevisiae.YEAST,37,(3)
MLA：
Xu, Xianyan,et al."PSK1 coordinates glucose metabolism and utilization and regulates energy-metabolism oscillation in Saccharomyces cerevisiae".YEAST 37..3(2020):261-268
