机构：[1]Department of Biochemistry 2, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi 409-3898, Japan[2]Beijing Institute of Otolaryngology, Beijing Tongren Hospital, Dongcheng District, Beijing 100730, China研究所耳鼻咽喉科研究所首都医科大学附属北京同仁医院首都医科大学附属同仁医院[3]Department of Pediatrics, Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China
Ultradian clock-coupled respiratory oscillation (UCRO) in an aerobic continuous culture of Saccharomyces cerevisiae S288C is principally regulated by control of certain redox reactions of energy metabolism. It is also modulated by the metabolism of storage carbohydrates during adaptation to environmental change. However, the mechanism of cell sensing and response to environmental nutrients in UCRO is unknown. The purpose of the present study was to determine the role of PSK2 kinase in UCRO in yeast. S. cerevisiae in culture showed oscillation in PSK2 mRNA levels with a definite phase relationship to the respiratory oscillation. Furthermore, inactivation of Psk2 by gene disruption severely affected UCRO and its decline to undetectable levels within 2 days. In addition, the extracellular and intracellular glucose concentrations of PSK2 deletion mutants in culture were higher and lower, respectively, than those of the wild type. PSK2 mutant cells showed no alteration in redox state. Furthermore, the levels of storage carbohydrates such as glycogen and trehalose fluctuated in PSK2 mutants with attenuated amplitudes comparable to those in the wild type. The results indicated that PSK2 kinase is important for the uptake of glucose and regulation of storage-carbohydrate synthesis and hence the maintenance of an unperturbed continuously oscillating state. (C) 2011 Elsevier Inc. All rights reserved.
基金：
Grant-in-Aid for Scientific Research from the University of
Yamanashi
第一作者机构：[1]Department of Biochemistry 2, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi 409-3898, Japan[2]Beijing Institute of Otolaryngology, Beijing Tongren Hospital, Dongcheng District, Beijing 100730, China
通讯作者：
通讯机构：[1]Department of Biochemistry 2, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Yamanashi 409-3898, Japan[*1]Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Biochem 2, Chuo Ku, 1110 Shimokato, Yamanashi 4093898, Japan
推荐引用方式(GB/T 7714)：
Ouyang Yuhui,Xu Qinghong,Mitsui Kazuhiro,et al.PSK2 coordinates glucose metabolism and utilization to maintain Ultradian clock-coupled respiratory oscillation in Saccharomyces cerevisiae yeast[J].ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS.2011,509(1):52-58.doi:10.1016/j.abb.2011.02.005.
APA：
Ouyang, Yuhui,Xu, Qinghong,Mitsui, Kazuhiro,Motizuki, Mitsuyoshi&Xu, Zhaojun.(2011).PSK2 coordinates glucose metabolism and utilization to maintain Ultradian clock-coupled respiratory oscillation in Saccharomyces cerevisiae yeast.ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS,509,(1)
MLA：
Ouyang, Yuhui,et al."PSK2 coordinates glucose metabolism and utilization to maintain Ultradian clock-coupled respiratory oscillation in Saccharomyces cerevisiae yeast".ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 509..1(2011):52-58
