Background. The purpose of this study was to investigate the presence of CD4(+)CD25(+)FOXP3(+) regulatory T (Treg) cells both in peripheral blood and local tumors in patients with nasal inverted papilloma (NIP). Methods. By using flow cytometry, the frequencies of CD4(+)CD25(+)FOXP3(+) Treg cells in both peripheral blood and tissues from 18 patients with NIP and 8 control subjects were determined. CCL22 and CCL17 proteins in NIP tumors were analyzed by using enzyme-linked immunosorbent assay. The suppressive capacity of Treg cells was estimated by WST-8 and enzyme-linked immunosorbent assay (ELISA; interferon-gamma [IFN-gamma] and interleukin-4 [IL-4]) analysis. Results. Patients with NIP showed increased CD4(+)CD25(+)FOXP3(+) Treg cell frequencies in tumor tissues and CD4(+) T cell fraction rather than in peripheral blood. CCL22 increased in NIP tumors. Phytohemagglutinin (PHA)-induced proliferation and cytokine production of CD4(+)CD25(-) T cells were suppressed equally well by CD4(+)CD25(high) cells from both patients with NIP and controls. Conclusion. Our study demonstrated the increased frequencies of CD4(+)CD25(+)FOXP3(+) Treg cells in NIP tumors, which might be influenced by CCL22. (C) 2010 Wiley Periodicals, Inc. Head Neck 33: 1005-1012, 2011
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30872846]; Beijing Natural Science FoundationBeijing Natural Science Foundation [7072017]; Beijing Education Committee Foundation [KZ200910025008]
第一作者机构：[1]Capital Med Univ, Minist Educ China, Beijing Inst Otorhinolaryngol, Key Lab Otolaryngol Head & Neck Surg, Beijing 100005, Peoples R China[2]Capital Med Univ, Affiliated Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, Beijing 100730, Peoples R China
通讯作者：
通讯机构：[1]Capital Med Univ, Minist Educ China, Beijing Inst Otorhinolaryngol, Key Lab Otolaryngol Head & Neck Surg, Beijing 100005, Peoples R China[2]Capital Med Univ, Affiliated Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, Beijing 100730, Peoples R China
推荐引用方式(GB/T 7714)：
Gu Yili,Wang Chengshuo,Han Demin,et al.INCREASED FREQUENCIES OF CD4(+)CD25(+)FOXP3(+) REGULATORY T CELLS IN HUMAN NASAL INVERTED PAPILLOMA[J].HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK.2011,33(7):1005-1012.doi:10.1002/hed.21581.
APA：
Gu, Yili,Wang, Chengshuo,Han, Demin&Zhang, Luo.(2011).INCREASED FREQUENCIES OF CD4(+)CD25(+)FOXP3(+) REGULATORY T CELLS IN HUMAN NASAL INVERTED PAPILLOMA.HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK,33,(7)
MLA：
Gu, Yili,et al."INCREASED FREQUENCIES OF CD4(+)CD25(+)FOXP3(+) REGULATORY T CELLS IN HUMAN NASAL INVERTED PAPILLOMA".HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK 33..7(2011):1005-1012
