机构：[1]Department of Radiology and Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA[2]Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA[3]Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA[4]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, and Bejing Ophthalmology and Visual Science Key Lab, Beijing, China首都医科大学附属北京同仁医院首都医科大学附属同仁医院[5]Peking University Eye Center, Peking University Third Hospital, Beijing, China
Purpose: To investigate whether systemic administration of methamphetamine (METH) induces retinal damage in CD1 mice. Materials and Methods: Eighteen male CD1 mice were randomly assigned to three groups, six mice per group: Group 1 receiving a single dose of 40 mg/kg METH, Group 2 receiving four doses of 10 mg/kg METH, and Group 3 (control) receiving 40 mg/kg 0.9% NaCl solution. METH and NaCl were administered by intraperitoneal injection. Immunostaining of glial fibrillary acidic protein (GFAP), S-100 for astrocytes and Muller cells, CD11b for microglia, and tyrosine hydroxylase (TH) and TUNEL labeling for apoptotic cell death were performed on the retinal sections on day 1 and day 7 post-exposure. Results: GFAP and S-100 immunoreactivity was observed in Group 1 mice. CD11b+ cells in Group 1mice showed more intensely stained shorter and thicker cellular processes than Groups 2 and 3, indicating activated microglia in the mice exposed to large-dose METH. No significant difference in TH level was seen among the three groups. TUNEL labeling did not reveal positive cells in the retinas of any of the 18 CD1 mice. Conclusions: A single large dose of METH induces an increase in short-term protein expression of GFAP and S-100 and in microglial activation. The results suggest that METH has a neurotoxic effect on CD1 mouse retina.
基金：
This work was supported by a grant from NIH/NIDA
DA-15020 (SL).
第一作者机构：[1]Department of Radiology and Wilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
通讯作者：
推荐引用方式(GB/T 7714)：
Lai Hong,Zhang Cheng,Wang Lin,et al.Toxic Effect of Methamphetamine on the Retina of CD1 Mice[J].CURRENT EYE RESEARCH.2009,34(9):785-790.doi:10.1080/02713680903079641.
APA：
Lai, Hong,Zhang, Cheng,Wang, Lin,Lai, Shenghan,Zeng, Huiyang&Tso, Mark O. M..(2009).Toxic Effect of Methamphetamine on the Retina of CD1 Mice.CURRENT EYE RESEARCH,34,(9)
MLA：
Lai, Hong,et al."Toxic Effect of Methamphetamine on the Retina of CD1 Mice".CURRENT EYE RESEARCH 34..9(2009):785-790
