机构：[1]National Laboratory of Medical Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences and Peking Union Medical College, National Human Genome Center, Beijing[2]Beijing Tongren hospital, Capital University of Medical Science, Chong Nei Street, Beijing, China首都医科大学附属北京同仁医院首都医科大学附属同仁医院
In a yeast two-hybrid screen, we identified the microtubule-destabilizing protein SCG10 as a potential effector protein of BRI3. The association was verified using GST pull-down, Co-IP, and their perinuclear co-localization. The analysis of in vitro microtubule polymerization/depolymerization showed that the binding of BRI3 to SCG10 effectively blocked the ability of SCG10 to induce microtubule disassembly, as determined by turbidimetric assays. In intact PC12 cells, BRI3 exhibited the ability to stabilize the microtubule network and attenuate the microtubule-destabilizing activity of SCG10. Furthermore, co-expression of BRI3 with SCG 10 attenuated SCG10-mediated PCII 2 cell neurite outgrowth induced by NGF. These results identify a novel connection between a neuron-specific BRI protein and the cytoskeletal network, suggesting possible roles of BRI3 in the process of neuronal differentiation.
基金：
This work was supported by grants from the National High
Technology Research and Development Program (“863”No.
2006AA02Z137); the National Program for the Key Basic
Research Project (“973”No. 2004CB518604, 2005CB522507,
and 2006CB504100); and the National Sciences Foundation of
China (No 30421003 and 30430200).
第一作者机构：[1]National Laboratory of Medical Molecular Biology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences and Peking Union Medical College, National Human Genome Center, Beijing
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Gong Yanhua,Wu Jing,Qiang Hua,et al.BRI3 associates with SCG10 and attenuates NGF-induced neurite outgrowth in PC12 cells[J].BMB REPORTS.2008,41(4):287-293.doi:10.5483/BMBRep.2008.41.4.287.
APA：
Gong, Yanhua,Wu, Jing,Qiang, Hua,Liu, Ben,Chi, Zhikai...&Yuan, Jiangang.(2008).BRI3 associates with SCG10 and attenuates NGF-induced neurite outgrowth in PC12 cells.BMB REPORTS,41,(4)
MLA：
Gong, Yanhua,et al."BRI3 associates with SCG10 and attenuates NGF-induced neurite outgrowth in PC12 cells".BMB REPORTS 41..4(2008):287-293
