Objective: To evaluate whether the nailfold microcirculation is associated with retinal microcirculation in healthy subjects. Methods: Fifty subjects without systematic and ocular diseases were enrolled. Thickness of peripapillary retinal nerve fiber layer (RNFL), vessel density (VD) of radial peripapillary capillaries (RPCs), and superficial capillary VD in macular zone were measured with optical coherence tomography angiography (OCTA) in left eyes. Nailfold microcirculation, including capillary density, avascular zones, dilated capillaries, and hemorrhages was examined on the fourth digit of each subject's non-dominant (left) hand with nailfold capillaroscopy (NFC). Results: After adjustment for relatively systemic factors, multivariate regression analyses showed a significant direct relationship between RNFL thickness and nailfold capillary density (OR=1.09;p=0.046). RNFL thickness and RPCs VD were negatively correlated with nailfold avascular zones (OR=0.855;p=0.007; OR=0.596;p=0.010). Superficial VD of parafovea was negatively associated with dilated nailfold capillaries (OR=0.794;p=0.012). Conclusion: In healthy subjects, nailfold capillary lower density and abnormalities are associated with reduced RNFL thickness and retinal VD. The results provide a theoretical foundation for relevant studies on ocular diseases with microvascular abnormalities and could contribute to pathogenesis understanding in the future. NFC and OCTA have the potential to identify risk factors and improve accuracy of the early diagnosis and treatment of ocular diseases, even systemic diseases with any microvascular component in clinical practice. Clinical Trail Registration: http://www.chictr.org.cn/index.aspx, identifier ChiCTR 1800017875.