To investigate the correlation between high-risk human papillomavirus infection and the expression of ER, PR, P53 and Ki67 in patients with different grades of cervical intraepithelial neoplasia. A retrospective study was conducted in which from June 2016 to June 2018, 140 specimens of cervical intraepithelial neoplasia were collected from the pathology department of the Beijing Tongren Hospital that included 40 specimens of cervical intraepithelial neoplasia1, 50 specimens of cervical intraepithelial neoplasia2 and 50 specimens of cervical intraepithelial neoplasia1. The expression of ER, PR, p53 and Ki67 were determined by immunohistochemistry. High-risk human papillomavirus infections were detected by polymerase chain reaction fluorescence quantification and were given the correlation analysis. Results of the 140 specimens indicated the rates of human papillomavirus16 and human papillomavirus18 in cervical intraepithelial neoplasia1 specimens were 27.5 and 25.0 % respectively, in cervical intraepithelial neoplasia2 specimens were 64.0 and 60.0 %, respectively and in cervical intraepithelial neoplasia3 specimens were 90.0 and 92.0 % respectively, the difference were statistically significant (p<0.05). There was no significant correlation between human papillomavirus 16 and human papillomavirus 18 positive rate and patient age, tissue differentiation, and tumour size. With the increasing cervical intraepithelial neoplasia grade, the rate of ER, PR, p53 and Ki67 expression in specimens was also increased significantly (p<0.05). Pearson correlation analysis showed there were positive correlation between the rates of human papillomavirus 16 and human papillomavirus 18 and the rates of ER, PR, p53 and Ki67 expression (p<0.05). In conclusion with increasing cervical intraepithelial neoplasia level, rate of high-risk human papillomavirus infection increased along with increased rate of expression of ER, PR, p53 and Ki67.