详情页 - 首都医科大学附属北京同仁医院知识库

当前位置：首页 > 详情页

Therapeutic potential of Rho-associated kinase inhibitor Y27632 in corneal endothelial dysfunction: an in vitro and in vivo study

1| 认领 | 导出 | 链接全文 |

文献详情

资源类型：

WOS体系：

Pubmed体系：

收录情况： ◇ SCIE

作者：

机构： [1]Shanxi Eye Hosp, Taiyuan 030002, Shanxi, Peoples R China [2]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Ophthalmol & Visual Sci Key Lab, Dongjiaominxiang 1, Beijing 100730, Peoples R China

出处：

DOI：

ISSN：

关键词： corneal endothelial dysfunction Rho kinase inhibitor Y-27632 porcine corneal endothelial cells cell proliferation

摘要：

AIM: To investigate the effects of a selective inhibitor of Rho-associated kinase (ROCK), Y-27632, on inbred Wuzhishan porcine corneal endothelial cells (PCECs) in vitro and in vivo studies. METHODS: Primary PCECs were trypsinized from Wuzhishan miniature porcine corneal tissues. The optimal concentration of Y-27632 on PCECs was determined through MTT and 5-ethynyl-2'-deoxyuridine (EdU)-labeling assays. Seven New Zealand rabbits were used as a corneal endothelial dysfunction model, and a PCECs suspension supplemented with Y-27632 was injected into the anterior chamber of the rabbits. The progression of rabbit corneal opacity and edema were observed by slit lamp examination. The rabbits were sacrificed, and rabbit globes were enucleated for trypan blue-alizarin red staining, hematoxylineosin staining, and immunofluorescence analysis. RESULTS: Administration of 100 mu mol/L Y-27632 facilitated PCECs' proliferation obviously. The rabbit corneas injected with PCECs suspension and 100 mu mol/L Y-27632 were restored to transparency significantly after 14d. CONCLUSION: The 100 mu mol/L Y-27632 treatment improves PCECs' proliferation significantly. And our results suggest that Y-27632 and PCECs can be used to treat corneal endothelial dysfunction.

基金：

语种：

WOS：

PubmedID：

中科院(CAS)分区：

出版当年[2020]版：

大类 | 3 区医学

小类 | 3 区眼科学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区眼科学

JCR分区：

出版当年[2019]版：

Q4 OPHTHALMOLOGY

最新[2023]版：

Q2 OPHTHALMOLOGY

影响因子： 1.9 最新[2023版] 1.8 最新五年平均 1.33 出版当年[2019版] 1.371 出版当年五年平均 1.189 出版前一年[2018版] 1.779 出版后一年[2020版]

第一作者：

第一作者机构： [1]Shanxi Eye Hosp, Taiyuan 030002, Shanxi, Peoples R China [2]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Ophthalmol & Visual Sci Key Lab, Dongjiaominxiang 1, Beijing 100730, Peoples R China

通讯作者：

通讯机构： [2]Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing Ophthalmol & Visual Sci Key Lab, Dongjiaominxiang 1, Beijing 100730, Peoples R China [*1]Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Laboratory, Dongjiaominxiang 1#, Dongcheng District, Beijing 100730, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]Rho-kinase inhibitor alleviates CD4+T cell mediated corneal graft rejection by modulating its STAT3 and STAT5 activation [2]Glass fragment in the anterior chamber caused corneal endothelial dysfunction eight years after penetrating trauma [3]ROCK inhibitor enhances mitochondrial transfer via tunneling nanotubes in retinal pigment epithelium [4]Inhibition of RhoA/Rho-kinase pathway suppresses the expression of extracellular matrix induced by CTGF or TGF-β in ARPE-19 [5]Givinostat inhibition of hepatic stellate cell proliferation and protein acetylation [6]Upregulation of BTF3 affects the proliferation, apoptosis, and cell cycle regulation in hypopharyngeal squamous cell carcinoma [7]Loss of FAM172A gene prompts cell proliferation in liver regeneration [8]Givinostat inhibition of hepatic stellate cell proliferation and protein acetylation [9]Sonic hedgehog promotes mouse inner ear progenitor cell proliferation and hair cell generation in vitro [10]Gab3 is required for human colorectal cancer cell proliferation